The use of human papillomavirus DNA methylation in cervical intraepithelial neoplasia : A systematic review and meta-analysis

Visa fullständig post



Permalänk

http://hdl.handle.net/10138/309753

Citation

Bowden , S J , Kalliala , I , Veroniki , A A , Arbyn , M , Mitra , A , Lathouras , K , Mirabello , L , Chadeau-Hyam , M , Paraskevaidis , E , Flanagan , J M & Kyrgiou , M 2019 , ' The use of human papillomavirus DNA methylation in cervical intraepithelial neoplasia : A systematic review and meta-analysis ' , EBioMedicine , vol. 50 , pp. 246-259 . https://doi.org/10.1016/j.ebiom.2019.10.053

Titel: The use of human papillomavirus DNA methylation in cervical intraepithelial neoplasia : A systematic review and meta-analysis
Författare: Bowden, Sarah J.; Kalliala, Ilkka; Veroniki, Areti A.; Arbyn, Marc; Mitra, Anita; Lathouras, Kostas; Mirabello, Lisa; Chadeau-Hyam, Marc; Paraskevaidis, Evangelos; Flanagan, James M.; Kyrgiou, Maria
Upphovmannens organisation: Clinicum
Department of Obstetrics and Gynecology
HUS Gynecology and Obstetrics
University of Helsinki
Datum: 2019-12
Språk: eng
Sidantal: 14
Tillhör serie: EBioMedicine
ISSN: 2352-3964
DOI: https://doi.org/10.1016/j.ebiom.2019.10.053
Permanenta länken (URI): http://hdl.handle.net/10138/309753
Abstrakt: Background: Methylation of viral DNA has been proposed as a novel biomarker for triage of human papillomavirus (HPV) positive women at screening. This systematic review and meta-analysis aims to assess how methylation levels change with disease severity and to determine diagnostic test accuracy (DTA) in detecting high-grade cervical intra-epithelial neoplasia (CIN). Methods: We performed searches in MEDLINE, EMBASE and CENTRAL from inception to October 2019. Studies were eligible if they explored HPV methylation levels in HPV positive women. Data were extracted in duplicate and requested from authors where necessary. Random-effects models and a bivariate mixed-effects binary regression model were applied to determine pooled effect estimates. Findings: 44 studies with 8819 high-risk HPV positive women were eligible. The pooled estimates for positive methylation rate in HPV16 L1 gene were higher for high-grade CIN (>= CIN2/high-grade squamous intra-epithelial lesion (HSIL) (95% confidence interval (95%CI:72.7% (47 8-92.2))) vs. low-grade CIN (= CIN2/HSIL vs. = CIN2/HSIL vs. Interpretation: Higher HPV methylation is associated with increased disease severity, whilst HPV16 L1/L2 genes demonstrated high diagnostic accuracy to detect high-grade CIN in HPV16 positive women. Direct clinical use is limited by the need for a multi-genotype and standardised assays. Next-generation multiplex HPV sequencing assays are under development and allow potential for rapid, automated and low-cost methylation testing. (C) 2019 The Authors. Published by Elsevier B.V.
Subject: Human papillomavirus
DNA methylation
Cervical intraepithelial neoplasia
Cervical screening
Meta-analysis
E2 BINDING-SITES
RESOLUTION MELTING ANALYSIS
HPV 16 METHYLATION
L1 GENE
QUANTITATIVE MEASUREMENT
POTENTIAL BIOMARKER
CPG METHYLATION
MESSENGER-RNA
TYPE-16 DNA
E6 GENE
3121 General medicine, internal medicine and other clinical medicine
3123 Gynaecology and paediatrics
Referentgranskad: Ja
Licens: cc_by
Användningsbegränsning: openAccess
Parallelpublicerad version: publishedVersion


Filer under denna titel

Totalt antal nerladdningar: Laddar...

Filer Storlek Format Granska
1_s2.0_S2352396419307285_main.pdf 2.556Mb PDF Granska/Öppna

Detta dokument registreras i samling:

Visa fullständig post