Feasibility of ultra-performance liquid chromatography-ion mobility-time-of-flight mass spectrometry in analyzing oxysterols

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Kylli , P , Hankemeier , T & Kostiainen , R 2017 , ' Feasibility of ultra-performance liquid chromatography-ion mobility-time-of-flight mass spectrometry in analyzing oxysterols ' , Journal of Chromatography. A , vol. 1487 , pp. 147-152 . https://doi.org/10.1016/j.chroma.2017.01.039

Title: Feasibility of ultra-performance liquid chromatography-ion mobility-time-of-flight mass spectrometry in analyzing oxysterols
Author: Kylli, Petri; Hankemeier, Thomas; Kostiainen, Risto
Contributor organization: Faculty of Pharmacy
Division of Pharmaceutical Chemistry and Technology
University of Helsinki
Risto Kostiainen / Principal Investigator
Drug Research Program
Date: 2017-03-03
Language: eng
Number of pages: 6
Belongs to series: Journal of Chromatography. A
ISSN: 0021-9673
DOI: https://doi.org/10.1016/j.chroma.2017.01.039
URI: http://hdl.handle.net/10138/309761
Abstract: Oxysterols are oxygenated cholesterols that are important in many cell functions and they may also be indicative of certain diseases. The purpose of this work was to study the feasibility of ultra-performance liquid chromatography-ion mobility-time-of-flight mass spectrometry (UPLC-IM-TOFMS) using traveling wave cell in analyzing oxysterols and especially their isomers in biological samples. Oxysterols were analyzed as their p-toluenesulfonyl isocyanate derivatives, which improved the separation of isomeric oxysterols by ion mobility and ionization efficiency in the electrospray ionization step. The UPLC-IM-TOFMS method was shown to be fast and to provide good quantitative performance. The feasibility of the method was demonstrated in the analyses of oxysterols in fibroblast cell samples. (C) 2017 Elsevier B.V. All rights reserved.
Subject: Liquid-chromatography-ion-mobilitymass-spectrometry,oxysterol
Oxysterol isomers
Derivatization
Fibroblast cell samples
HUMAN PLASMA
CHOLESTEROL-METABOLISM
STEM-CELLS
IN-VIVO
MS
SEPARATION
ISOMERS
24S-HYDROXYCHOLESTEROL
DIFFERENTIATION
QUANTIFICATION
1182 Biochemistry, cell and molecular biology
116 Chemical sciences
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion


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