CYPome of the conifer pathogen Heterobasidion irregulare : Inventory, phylogeny, and transcriptional analysis of the response to biocontrol

Show full item record



Permalink

http://hdl.handle.net/10138/309849

Citation

Mgbeahuruike , A C , Kovalchuk , A , Ubhayasekera , W , Nelson , D R & Yadav , J S 2017 , ' CYPome of the conifer pathogen Heterobasidion irregulare : Inventory, phylogeny, and transcriptional analysis of the response to biocontrol ' , Fungal Biology , vol. 121 , no. 2 , pp. 158-171 . https://doi.org/10.1016/j.funbio.2016.11.006

Title: CYPome of the conifer pathogen Heterobasidion irregulare : Inventory, phylogeny, and transcriptional analysis of the response to biocontrol
Author: Mgbeahuruike, Anthony C.; Kovalchuk, Andriy; Ubhayasekera, Wimal; Nelson, David R.; Yadav, Jagjit S.
Contributor: University of Helsinki, Department of Forest Sciences
Date: 2017-02
Language: eng
Number of pages: 14
Belongs to series: Fungal Biology
ISSN: 1878-6146
URI: http://hdl.handle.net/10138/309849
Abstract: The molecular mechanisms underlying the interaction of the pathogen, Heterobasidion annosum s.l., the conifer tree and the biocontrol fungus, Phlebiopsis gigantea have not been fully elucidated. Members of the cytochrome P450 (CYP) protein family may contribute to the detoxification of components of chemical defence of conifer trees by H. annosum during infection. Additionally, they may also be involved in the interaction between H. annosum and P. gigantea. A genome-wide analysis of CYPs in Heterobasidion irregulare was carried out alongside gene expression studies. According to the Standardized CYP Nomenclature criteria, the H. irregulare genome has 121 CYP genes and 17 CYP pseudogenes classified into 11 clans, 35 families, and 64 subfamilies. Tandem CYP arrays originating from gene duplications and belonging to the same family and subfamily were found. Phylogenetic analysis showed that all the families of H. irregulare CYPs were monophyletic groups except for the family CYP5144. Microarray analysis revealed the transcriptional pattern for 130 transcripts of CYP-encoding genes during growth on culture filtrate produced by P. gigantea. The high level of P450 gene diversity identified in this study could result from extensive gene duplications presumably caused by the high metabolic demands of H. irregulare in its ecological niches. (C) 2016 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
Subject: Gene expression
Genome
Heterobasidion annosum s.l.
Homology modelling
P450
FUNGUS PHANEROCHAETE-CHRYSOSPORIUM
NORWAY SPRUCE STUMPS
PHLEBIOPSIS-GIGANTEA
CYTOCHROME-P450 GENES
PLEUROTUS-OSTREATUS
FOREST PATHOGEN
GENOME SEQUENCE
STORAGE FUNGI
WOOD DECAY
ANNOSUM
1183 Plant biology, microbiology, virology
1182 Biochemistry, cell and molecular biology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
mgbeahuruike2016.pdf 3.119Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record