Structural Imaging of Native Cryo-Preserved Secondary Cell Walls Reveals the Presence of Macrofibrils and Their Formation Requires Normal Cellulose, Lignin and Xylan Biosynthesis

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dc.contributor.author Lyczakowski, Jan J.
dc.contributor.author Bourdon, Matthieu
dc.contributor.author Terrett, Oliver M.
dc.contributor.author Helariutta, Ykä
dc.contributor.author Wightman, Raymond
dc.contributor.author Dupree, Paul
dc.date.accessioned 2020-02-04T08:32:02Z
dc.date.available 2020-02-04T08:32:02Z
dc.date.issued 2019-10-23
dc.identifier.citation Lyczakowski , J J , Bourdon , M , Terrett , O M , Helariutta , Y , Wightman , R & Dupree , P 2019 , ' Structural Imaging of Native Cryo-Preserved Secondary Cell Walls Reveals the Presence of Macrofibrils and Their Formation Requires Normal Cellulose, Lignin and Xylan Biosynthesis ' , Frontiers in plant science , vol. 10 , 1398 . https://doi.org/10.3389/fpls.2019.01398
dc.identifier.other PURE: 131398172
dc.identifier.other PURE UUID: 1e29cdd7-2c79-4f22-bbdb-45537b458561
dc.identifier.other WOS: 000497585100001
dc.identifier.other ORCID: /0000-0002-7287-8459/work/68613928
dc.identifier.uri http://hdl.handle.net/10138/310944
dc.description.abstract The woody secondary cell walls of plants are the largest repository of renewable carbon biopolymers on the planet. These walls are made principally from cellulose and hemicelluloses and are impregnated with lignin. Despite their importance as the main load bearing structure for plant growth, as well as their industrial importance as both a material and energy source, the precise arrangement of these constituents within the cell wall is not yet fully understood. We have adapted low temperature scanning electron microscopy (cryo-SEM) for imaging the nanoscale architecture of angiosperm and gymnosperm cell walls in their native hydrated state. Our work confirms that cell wall macrofibrils, cylindrical structures with a diameter exceeding 10 nm, are a common feature of the native hardwood and softwood samples. We have observed these same structures in Arabidopsis thaliana secondary cell walls, enabling macrofibrils to be compared between mutant lines that are perturbed in cellulose, hemicellulose, and lignin formation. Our analysis indicates that the macrofibrils in Arabidopsis cell walls are dependent upon the proper biosynthesis, or composed, of cellulose, xylan, and lignin. This study establishes that cryo-SEM is a useful additional approach for investigating the native nanoscale architecture and composition of hardwood and softwood secondary cell walls and demonstrates the applicability of Arabidopsis genetic resources to relate fibril structure with wall composition and biosynthesis. en
dc.format.extent 14
dc.language.iso eng
dc.relation.ispartof Frontiers in plant science
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject scanning electron microscopy
dc.subject cell walls
dc.subject macrofibrils
dc.subject cellulose
dc.subject xylan
dc.subject lignin
dc.subject softwood
dc.subject hardwood
dc.subject WOOD-DENSITY
dc.subject MICROFIBRILS
dc.subject POLYSACCHARIDES
dc.subject SUBSTITUTION
dc.subject GLUCURONOXYLAN
dc.subject DISRUPTION
dc.subject DEPOSITION
dc.subject PATTERNS
dc.subject ENCODES
dc.subject SPRUCE
dc.subject 1183 Plant biology, microbiology, virology
dc.title Structural Imaging of Native Cryo-Preserved Secondary Cell Walls Reveals the Presence of Macrofibrils and Their Formation Requires Normal Cellulose, Lignin and Xylan Biosynthesis en
dc.type Article
dc.contributor.organization Viikki Plant Science Centre (ViPS)
dc.contributor.organization Yrjö Helariutta / Principal Investigator
dc.contributor.organization Institute of Biotechnology
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.3389/fpls.2019.01398
dc.relation.issn 1664-462X
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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