Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres

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Mohlin , S , Kunttas , E , Persson , C U , Abdel-Haq , R , Castillo , A , Murko , C , Bronner , M E & Kerosuo , L 2019 , ' Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres ' , Developmental Biology , vol. 447 , no. 2 , pp. 137-146 .

Title: Maintaining multipotent trunk neural crest stem cells as self-renewing crestospheres
Author: Mohlin, Sofie; Kunttas, Ezgi; Persson, Camilla U.; Abdel-Haq, Reem; Castillo, Aldo; Murko, Christina; Bronner, Marianne E.; Kerosuo, Laura
Other contributor: University of Helsinki, Department of Biochemistry and Developmental Biology

Date: 2019-03-15
Language: eng
Number of pages: 10
Belongs to series: Developmental Biology
ISSN: 0012-1606
Abstract: Neural crest cells have broad migratory and differentiative ability that differs according to their axial level of origin. However, their transient nature has limited understanding of their stem cell and self-renewal properties. While an in vitro culture method has made it possible to maintain cranial neural crest cells as self-renewing multipotent crestospheres (Kerosuo et al., 2015), these same conditions failed to preserve trunk neural crest in a stem-like state. Here we optimize culture conditions for maintenance of avian trunk crestospheres, comprised of both neural crest stem and progenitor cells. Our trunk-derived crestospheres are multipotent and display self-renewal capacity over several weeks. Trunk crestospheres display elevated expression of neural crest cell markers as compared to those characteristic of ventrolateral neural tube or mesodermal fates. Moreover, trunk crestospheres express increased levels of trunk neural crest-enriched markers as compared to cranial crestospheres. Finally, we use lentiviral transduction as a tool to manipulate gene expression in trunk crestospheres. Taken together, this method enables long-term in vitro maintenance and manipulation of multipotent trunk neural crest cells in a premigratory stem or early progenitor state. Trunk crestospheres are a valuable resource for probing mechanisms underlying neural crest sternness and lineage decisions as well as accompanying diseases.
Subject: Crestospheres
Neural crest stem cells
Trunk neural crest
Stem cell maintenance
Multipotency, self-renewal
1182 Biochemistry, cell and molecular biology
1184 Genetics, developmental biology, physiology

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