Metabarcoding gastrointestinal nematodes in sympatric endemic and non-endemic species in Ranomafana National Park, Madagascar

Show full item record



Permalink

http://hdl.handle.net/10138/311338

Citation

Aivelo , T J E , Medlar , A J , Löytynoja , A P , Laakkonen , J T & Jernvall , J T 2018 , ' Metabarcoding gastrointestinal nematodes in sympatric endemic and non-endemic species in Ranomafana National Park, Madagascar ' , International Journal of Primatology , vol. 39 , no. 1 , pp. 49–64 . https://doi.org/10.1007/s10764-017-0010-x

Title: Metabarcoding gastrointestinal nematodes in sympatric endemic and non-endemic species in Ranomafana National Park, Madagascar
Author: Aivelo, Tuomas Juho Eero; Medlar, Alan John; Löytynoja, Ari Pekka; Laakkonen, Juha Tapio; Jernvall, Jukka Tapani
Contributor organization: Institute of Biotechnology
Biosciences
Ari Pekka Löytynoja / Principal Investigator
Bioinformatics
Departments of Faculty of Veterinary Medicine
Veterinary Biosciences
Veterinary Anatomy and Developmental Biology
Jukka Jernvall / Principal Investigator
Helsinki Institute of Sustainability Science (HELSUS)
Computational genomics
Global Change and Conservation Lab
Teachers' Academy
Date: 2018-02
Language: eng
Number of pages: 16
Belongs to series: International Journal of Primatology
ISSN: 0164-0291
DOI: https://doi.org/10.1007/s10764-017-0010-x
URI: http://hdl.handle.net/10138/311338
Abstract: Sympatric species are known to host the same parasites species. Nevertheless, surveys examining parasite assemblages in sympatric species are rare. To understand how parasite assemblages in sympatric host species differ in a given locality, we used a noninvasive identification method based on high-throughput sequencing. We collected fecal samples from sympatric species in Ranomafana National Park, Madagascar, from September to December in 2010, 2011, and 2012 and identified their parasites by metabarcoding, sequencing a region of the small ribosomal subunit (18S) gene. Our survey included 11 host species, including endemic primates, rodents, frogs, gastropods, and nonendemic rats and dogs. We collected 872 samples, of which 571 contained nematodes and 249 were successfully sequenced. We identified nine putative species of parasites, although their correspondence to actual parasite species is not clear as the resolution of the marker gene differs between nematode clades. For the host species that we successfully sampled with 10 or more positive occurrences of nematodes, i.e., mouse lemurs (Microcebus rufus), black rats (Rattus rattus), and frogs (Anura), the parasite assemblage compositions differed significantly among host species, sampling sites, and sampling years. Our metabarcoding method shows promise in interrogating parasite assemblages in sympatric host species and our results emphasize the importance of choosing marker regions for parasite identification accuracy.
Subject: 1181 Ecology, evolutionary biology
Invasive species
Lemurs
Metabarcoding
Noninvasive sampling
Parasites
ALOUATTA-PALLIATA-MEXICANA
NONINVASIVE ASSESSMENT
RAIN-FORESTS
DNA BARCODE
PARASITES
BIODIVERSITY
TRANSMISSION
COMMUNITIES
POPULATIONS
DIVERSITY
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: acceptedVersion


Files in this item

Total number of downloads: Loading...

Files Size Format View
barcoding_ijp_r2.pdf 128.4Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record