Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters

Show full item record



Permalink

http://hdl.handle.net/10138/311358

Citation

Falgenhauer , L , Schwengers , O , Schmiedel , J , Baars , C , Lambrecht , O , Hess , S , Berendonk , T U , Falgenhauer , J , Chakraborty , T & Imirzalioglu , C 2019 , ' Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters ' , Frontiers in Microbiology , vol. 10 , 2779 . https://doi.org/10.3389/fmicb.2019.02779

Title: Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters
Author: Falgenhauer, Linda; Schwengers, Oliver; Schmiedel, Judith; Baars, Christian; Lambrecht, Oda; Hess, Stefanie; Berendonk, Thomas U.; Falgenhauer, Jane; Chakraborty, Trinad; Imirzalioglu, Can
Other contributor: University of Helsinki, Department of Microbiology
Date: 2019-11-29
Language: eng
Number of pages: 11
Belongs to series: Frontiers in Microbiology
ISSN: 1664-302X
DOI: https://doi.org/10.3389/fmicb.2019.02779
URI: http://hdl.handle.net/10138/311358
Abstract: Water is considered to play a role in the dissemination of antibiotic-resistant Gram-negative bacteria including those encoding Extended-spectrum beta-lactamases (ESBL) and carbapenemases. To investigate the role of water for their spread in more detail, we characterized ESBL/Carbapenemase-producing bacteria from surface water and sediment samples using phenotypic and genotypic approaches. ESBL/Carbapenemase-producing isolates were obtained from water/sediment samples. Species and antibiotic resistance were determined. A subset of these isolates (n = 33) was whole-genome-sequenced and analyzed for the presence of antibiotic resistance genes and virulence determinants. Their relatedness to isolates associated with human infections was investigated using multilocus sequence type and cgMLST-based analysis. Eighty-nine percent of the isolates comprised of clinically relevant species. Fifty-eight percent exhibited a multidrug-resistance phenotype. Two isolates harbored the mobile colistin resistance gene mcr-1. One carbapenemase-producing isolate identified as Enterobacter kobei harbored bla(VIM-)(1). Two Escherichia coli isolates had sequence types (ST) associated with human infections (ST131 and ST1485) and a Klebsiella pneumoniae isolate was classified as hypervirulent. A multidrug-resistant (MDR) Pseudomonas aeruginosa isolate encoding known virulence genes associated with severe lung infections in cystic fibrosis patients was also detected. The presence of MDR and clinically relevant isolates in recreational and surface water underlines the role of aquatic environments as both reservoirs and hot spots for MDR bacteria. Future assessment of water quality should include the examination of the multidrug resistance of clinically relevant bacterial species and thus provide an important link regarding the spread of MDR bacteria in a One Health context.
Subject: ESBL
surface water
WGS (whole genome sequencing)
MCR-1
clinical isolate
CARBAPENEMASE-PRODUCING ENTEROBACTERIACEAE
ESCHERICHIA-COLI
EXTENDED-SPECTRUM
KLEBSIELLA-PNEUMONIAE
PSEUDOMONAS-AERUGINOSA
HIGH PREVALENCE
ANIMALS
ENVIRONMENT
VIRULENCE
SAMPLES
1183 Plant biology, microbiology, virology
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
fmicb_10_02779.pdf 2.560Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record