Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters

Show simple item record Falgenhauer, Linda Schwengers, Oliver Schmiedel, Judith Baars, Christian Lambrecht, Oda Hess, Stefanie Berendonk, Thomas U. Falgenhauer, Jane Chakraborty, Trinad Imirzalioglu, Can 2020-02-10T16:26:05Z 2020-02-10T16:26:05Z 2019-11-29
dc.identifier.citation Falgenhauer , L , Schwengers , O , Schmiedel , J , Baars , C , Lambrecht , O , Hess , S , Berendonk , T U , Falgenhauer , J , Chakraborty , T & Imirzalioglu , C 2019 , ' Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters ' , Frontiers in Microbiology , vol. 10 , 2779 .
dc.identifier.other PURE: 131802666
dc.identifier.other PURE UUID: a693b531-f4c5-4c14-80d1-20d2cdf1b570
dc.identifier.other WOS: 000502808100001
dc.description.abstract Water is considered to play a role in the dissemination of antibiotic-resistant Gram-negative bacteria including those encoding Extended-spectrum beta-lactamases (ESBL) and carbapenemases. To investigate the role of water for their spread in more detail, we characterized ESBL/Carbapenemase-producing bacteria from surface water and sediment samples using phenotypic and genotypic approaches. ESBL/Carbapenemase-producing isolates were obtained from water/sediment samples. Species and antibiotic resistance were determined. A subset of these isolates (n = 33) was whole-genome-sequenced and analyzed for the presence of antibiotic resistance genes and virulence determinants. Their relatedness to isolates associated with human infections was investigated using multilocus sequence type and cgMLST-based analysis. Eighty-nine percent of the isolates comprised of clinically relevant species. Fifty-eight percent exhibited a multidrug-resistance phenotype. Two isolates harbored the mobile colistin resistance gene mcr-1. One carbapenemase-producing isolate identified as Enterobacter kobei harbored bla(VIM-)(1). Two Escherichia coli isolates had sequence types (ST) associated with human infections (ST131 and ST1485) and a Klebsiella pneumoniae isolate was classified as hypervirulent. A multidrug-resistant (MDR) Pseudomonas aeruginosa isolate encoding known virulence genes associated with severe lung infections in cystic fibrosis patients was also detected. The presence of MDR and clinically relevant isolates in recreational and surface water underlines the role of aquatic environments as both reservoirs and hot spots for MDR bacteria. Future assessment of water quality should include the examination of the multidrug resistance of clinically relevant bacterial species and thus provide an important link regarding the spread of MDR bacteria in a One Health context. en
dc.format.extent 11
dc.language.iso eng
dc.relation.ispartof Frontiers in Microbiology
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject ESBL
dc.subject surface water
dc.subject WGS (whole genome sequencing)
dc.subject MCR-1
dc.subject clinical isolate
dc.subject ANIMALS
dc.subject ENVIRONMENT
dc.subject VIRULENCE
dc.subject SAMPLES
dc.subject 1183 Plant biology, microbiology, virology
dc.title Multidrug-Resistant and Clinically Relevant Gram-Negative Bacteria Are Present in German Surface Waters en
dc.type Article
dc.contributor.organization Department of Microbiology
dc.description.reviewstatus Peer reviewed
dc.relation.issn 1664-302X
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
fmicb_10_02779.pdf 2.560Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record