No Tissue Expression of KRAS or BRAF Mutations in 61 Adult Patients Treated for Esophageal Atresia in Early Childhood

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http://hdl.handle.net/10138/311469

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Dang , K X , Ho , T , Sistonen , S , Koivusalo , A , Pakarinen , M , Rintala , R , Stenman , U-H , Orpana , A & Stenman , J 2018 , ' No Tissue Expression of KRAS or BRAF Mutations in 61 Adult Patients Treated for Esophageal Atresia in Early Childhood ' , European Journal of Pediatric Surgery , vol. 28 , no. 5 , pp. 413-419 . https://doi.org/10.1055/s-0037-1605346

Title: No Tissue Expression of KRAS or BRAF Mutations in 61 Adult Patients Treated for Esophageal Atresia in Early Childhood
Author: Dang, Kien Xuan; Ho, Tho; Sistonen, Saara; Koivusalo, Antti; Pakarinen, Mikko; Rintala, Risto; Stenman, Ulf-Hakan; Orpana, Arto; Stenman, Jakob
Contributor: University of Helsinki, Clinicum
University of Helsinki, Children's Hospital
University of Helsinki, Clinicum
University of Helsinki, Clinicum
University of Helsinki, Department of Clinical Chemistry and Hematology
University of Helsinki, HUSLAB
University of Helsinki, Institute for Molecular Medicine Finland
Date: 2018-10
Language: eng
Number of pages: 7
Belongs to series: European Journal of Pediatric Surgery
ISSN: 0939-7248
URI: http://hdl.handle.net/10138/311469
Abstract: Background Previous studies have reported an association among esophageal atresia (EA), Barrett's esophagus, and esophageal adenocarcinoma later in life. Objective The objective of the article is to evaluate KRAS and BRAF mutations as potential genetic markers for early detection of malignant transformation, we used an ultrasensitive technique to detect tissue expression of KRAS and BRAF mutations in endoscopic biopsies from 61 adult patients under follow-up after treatment for EA. Materials and Methods RNA was extracted from 112 fresh-frozen endoscopic tissue biopsies from 61 adult patients treated for EA in early childhood. RNA was reverse transcribed using the extendable blocking probe reverse transcription method. KRAS codons 12 and 13, as well as BRAF mutations were detected by quantitative polymerase chain reaction. Results No mutations of KRAS codon 12, KRAS codon 13, or BRAF were found in 112 endoscopic biopsy samples from 61 patients. Conclusion Despite the presence of histological findings indicating long-standing gastroesophageal reflux in 25%, as well as symptomatic gastroesophageal reflux in more than 40%, there was no detectable tissue expression of KRAS or BRAF mutations in this cohort of patients.
Subject: esophageal atresia
extendable blocking probe
KRAS
BRAF
SQUAMOUS-CELL-CARCINOMA
TERM-FOLLOW-UP
BARRETTS-ESOPHAGUS
TRACHEOESOPHAGEAL FISTULA
COLORECTAL-CANCER
RAS MUTATIONS
GASTROESOPHAGEAL-REFLUX
PRIMARY REPAIR
ADENOCARCINOMA
EVENTS
3123 Gynaecology and paediatrics
3126 Surgery, anesthesiology, intensive care, radiology
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