Tooth bioengineering from single cell suspensions

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http://hdl.handle.net/10138/311655

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Stratoulias , V & Michon , F 2019 , ' Tooth bioengineering from single cell suspensions ' , MethodsX , vol. 6 , pp. 2429-2438 . https://doi.org/10.1016/j.mex.2019.10.009

Title: Tooth bioengineering from single cell suspensions
Author: Stratoulias, Vassilis; Michon, Frederic
Contributor organization: Neuroscience Center
Institute of Biotechnology
Development and Physiology of the Eye
Date: 2019-10-17
Language: eng
Number of pages: 10
Belongs to series: MethodsX
ISSN: 2215-0161
DOI: https://doi.org/10.1016/j.mex.2019.10.009
URI: http://hdl.handle.net/10138/311655
Abstract: Recent advances in bioengineering and biomaterials, along with knowledge deriving from the fields of developmental biology and stem cell research, have rendered feasible functional replacement of full organs. Here, we describe the methodology for bioengineering a tooth, starting from embryonic epithelial and mesenchymal single cell suspensions. In addition, we describe the subsequent steps of processing this minute structure for use in applications such as histological examination, immunofluorescence and in situ hybridisation. This methodology can be used for any minute structure that needs to be used in paraffin blocks.•Detailed methodology for reproducible and reliable results•Extra step to ensure single cell populations•Subsequent minute structure processing for histological analysis
Subject: Tooth bioengineering
tooth
bioengineering
reconstruction
dental implant
epithelium
mesenchyme
313 Dentistry
318 Medical biotechnology
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: draft


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