Whole blood microRNA levels associate with glycemic status and correlate with target mRNAs in pathways important to type 2 diabetes

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http://hdl.handle.net/10138/311917

Lähdeviite

Mononen , N , Lyytikäinen , L-P , Seppälä , I , Mishra , P P , Juonala , M , Waldenberger , M , Klopp , N , Illig , T , Leiviskä , J , Loo , B-M , Laaksonen , R , Oksala , N , Kähönen , M , Hutri-Kähönen , N , Raitakari , O , Lehtimäki , T & Raitoharju , E 2019 , ' Whole blood microRNA levels associate with glycemic status and correlate with target mRNAs in pathways important to type 2 diabetes ' , Scientific Reports , vol. 9 , 8887 . https://doi.org/10.1038/s41598-019-43793-4

Julkaisun nimi: Whole blood microRNA levels associate with glycemic status and correlate with target mRNAs in pathways important to type 2 diabetes
Tekijä: Mononen, Nina; Lyytikäinen, Leo-Pekka; Seppälä, Ilkka; Mishra, Pashupati P.; Juonala, Markus; Waldenberger, Melanie; Klopp, Norman; Illig, Thomas; Leiviskä, Jaana; Loo, Britt-Marie; Laaksonen, Reijo; Oksala, Niku; Kähönen, Mika; Hutri-Kähönen, Nina; Raitakari, Olli; Lehtimäki, Terho; Raitoharju, Emma
Tekijän organisaatio: HUSLAB
Department of Diagnostics and Therapeutics
University of Helsinki
Päiväys: 2019-06-20
Kieli: eng
Sivumäärä: 14
Kuuluu julkaisusarjaan: Scientific Reports
ISSN: 2045-2322
DOI-tunniste: https://doi.org/10.1038/s41598-019-43793-4
URI: http://hdl.handle.net/10138/311917
Tiivistelmä: We analyzed the associations between whole blood microRNA profiles and the indices of glucose metabolism and impaired fasting glucose and examined whether the discovered microRNAs correlate with the expression of their mRNA targets. MicroRNA and gene expression profiling were performed for the Young Finns Study participants (n= 871). Glucose, insulin, and glycated hemoglobin (HbA1c) levels were measured, the insulin resistance index (HOMA2-IR) was calculated, and the glycemic status (normoglycemic [n = 534]/impaired fasting glucose [IFG] [n = 252]/type 2 diabetes [T2D] [n = 24]) determined. Levels of hsa-miR-144-5p, -122-5p, -148a-3p, -589-5p, and hsa-let-7a-5p associated with glycemic status. hsa-miR-144-5p and -148a-3p associated with glucose levels, while hsa-miR-144-5p, -122-5p, -184, and -339-3p associated with insulin levels and HOMA2-IR, and hsa-miR-148a-3p, -15b-3p, -93-3p, -146b-5p, -221-3p, -18a-3p, -642a-5p, and -181-2-3p associated with HbA1c levels. The targets of hsa-miR-146b-5p that correlated with its levels were enriched in inflammatory pathways, and the targets of hsa-miR-221-3p were enriched in insulin signaling and T2D pathways. These pathways showed indications of co-regulation by HbA1c-associated miRNAs. There were significant differences in the microRNA profiles associated with glucose, insulin, or HOMA-IR compared to those associated with HbA1c. The HbA1c-associated miRNAs also correlated with the expression of target mRNAs in pathways important to the development ofT2D.
Avainsanat: CARDIOVASCULAR RISK
DIFFERENTIAL EXPRESSION
POTENTIAL BIOMARKER
GLUCOSE
IDENTIFICATION
MELLITUS
PLASMA
COMPLICATIONS
REGULATOR
DISEASE
3111 Biomedicine
3121 General medicine, internal medicine and other clinical medicine
Vertaisarvioitu: Kyllä
Tekijänoikeustiedot: cc_by
Pääsyrajoitteet: openAccess
Rinnakkaistallennettu versio: publishedVersion


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