Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing

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http://hdl.handle.net/10138/311934

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Oliinyk , O S , Shemetov , A A , Pletnev , S , Shcherbakova , D M & Verkhusha , V V 2019 , ' Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing ' , Nature Communications , vol. 10 , 279 . https://doi.org/10.1038/s41467-018-08050-8

Title: Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing
Author: Oliinyk, Olena S.; Shemetov, Anton A.; Pletnev, Sergei; Shcherbakova, Daria M.; Verkhusha, Vladislav V.
Contributor: University of Helsinki, Department of Anatomy
University of Helsinki, Doctoral Programme in Biomedicine
Date: 2019-01-17
Language: eng
Number of pages: 13
Belongs to series: Nature Communications
ISSN: 2041-1723
URI: http://hdl.handle.net/10138/311934
Abstract: From a single domain of cyanobacteriochrome (CBCR) we developed a near-infrared (NIR) fluorescent protein (FP), termed miRFP670nano, with excitation at 645 nm and emission at 670 nm. This is the first CBCR-derived NIR FP evolved to efficiently bind endogenous biliverdin chromophore and brightly fluoresce in mammalian cells. miRFP670nano is a monomer with molecular weight of 17 kDa that is 2-fold smaller than bacterial phytochrome (BphP)-based NIR FPs and 1.6-fold smaller than GFP-like FPs. Crystal structure of the CBCR-based NIR FP with biliverdin reveals a molecular basis of its spectral and biochemical properties. Unlike BphP-derived NIR FPs, miRFP670nano is highly stable to denaturation and degradation and can be used as an internal protein tag. miRFP670nano is an effective FRET donor for red-shifted NIR FPs, enabling engineering NIR FRET biosensors spectrally compatible with GFP-like FPs and blue-green optogenetic tools. miRFP670nano unlocks a new source of diverse CBCR templates for NIR FPs.
Subject: KINASE
CHROMOPHORE
PHOTOCONVERSION
IDENTIFICATION
PHOTOCYCLES
REFINEMENT
RESONANCE
DYNAMICS
PHENIX
119 Other natural sciences
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