Plasma bradykinin concentrations during septic shock determined by a novel LC-MS/MS assay

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http://hdl.handle.net/10138/312116

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Lindström , M , Valkonen , M , Tohmola , N , Renkonen , R , Strandin , T , Vaheri , A & Itkonen , O 2019 , ' Plasma bradykinin concentrations during septic shock determined by a novel LC-MS/MS assay ' , Clinica Chimica Acta , vol. 493 , pp. 20-24 . https://doi.org/10.1016/j.cca.2019.02.023

Title: Plasma bradykinin concentrations during septic shock determined by a novel LC-MS/MS assay
Author: Lindström, Mikael; Valkonen, Miia; Tohmola, Niina; Renkonen, Risto; Strandin, Tomas; Vaheri, Antti; Itkonen, Outi
Contributor organization: Clinicum
University of Helsinki
HUSLAB
Faculty of Medicine
Anestesiologian yksikkö
HUS Perioperative, Intensive Care and Pain Medicine
Helsinki University Hospital Area
Risto Renkonen / Principal Investigator
Transplantation Laboratory
Medicum
Department of Virology
Viral Zoonosis Research Unit
Department of Clinical Chemistry and Hematology
Date: 2019-06
Language: eng
Number of pages: 5
Belongs to series: Clinica Chimica Acta
ISSN: 0009-8981
DOI: https://doi.org/10.1016/j.cca.2019.02.023
URI: http://hdl.handle.net/10138/312116
Abstract: Background: Bradykinin is an important mediator of inflammation and vascular permeability and could have an important role in the development of septic shock. Measurement of bradykinin by immunological methods may suffer from interference and lack of specificity. We developed and validated a liquid chromatography mass spectrometry assay (LC-MS/MS) for plasma bradykinin. Methods: We used plasma samples from healthy volunteers (n = 19) and patients with septic shock (n = 47). Stable isotope bradykinin internal standard was added to samples before solid-phase extraction and quantification by LC-MS/MS. Stability of bradykinin was studied for 12 months. Results: Our assay has good sensitivity (0.1 nmol/l) and a wide linear range (0.1-1000 nmol/1). Bradykinin added to plasma was stable for 12 months at -20 degrees C when a mixture of protease inhibitors was added at sampling but degraded during repeated freezing and thawing. Bradykinin concentration in plasma from septic shock patients (<0.1-0.6 nmol/l) did not change significantly during shock and recovery but differed slightly from that in healthy individuals (0.5-1.1 nmol/1). Conclusions: Our bradykinin assay was successfully used to determine bradykinin concentrations in plasma samples. Intensive care unit patients with septic shock had low concentrations of plasma bradykinin during both shock and recovery phases.
Subject: Bradykinin
Sepsis
Septic shock
LC-MS
Vascular permeability
KALLIKREIN-KININ SYSTEM
QUANTITATIVE ASSAY
ANTAGONIST
SEPSIS
PATHOPHYSIOLOGY
BLOOD
3111 Biomedicine
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion


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