Critical-sized cartilage defects in the equine carpus

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dc.contributor.author Salonius, Eve
dc.contributor.author Rieppo, Lassi
dc.contributor.author Nissi, Mikko J.
dc.contributor.author Pulkkinen, Hertta J.
dc.contributor.author Brommer, Harold
dc.contributor.author Bruenott, Anne
dc.contributor.author Silvast, Tuomo S.
dc.contributor.author Van Weeren, P. Rene
dc.contributor.author Muhonen, Virpi
dc.contributor.author Brama, Pieter A. J.
dc.contributor.author Kiviranta, Ilkka
dc.date.accessioned 2020-02-24T08:11:01Z
dc.date.available 2020-02-24T08:11:01Z
dc.date.issued 2019-03-04
dc.identifier.citation Salonius , E , Rieppo , L , Nissi , M J , Pulkkinen , H J , Brommer , H , Bruenott , A , Silvast , T S , Van Weeren , P R , Muhonen , V , Brama , P A J & Kiviranta , I 2019 , ' Critical-sized cartilage defects in the equine carpus ' , Connective Tissue Research , vol. 60 , no. 2 , pp. 95-106 . https://doi.org/10.1080/03008207.2018.1455670
dc.identifier.other PURE: 123877514
dc.identifier.other PURE UUID: 29e264ee-29cd-488a-8898-dc66f9251b12
dc.identifier.other WOS: 000461646700003
dc.identifier.other Scopus: 85045237066
dc.identifier.uri http://hdl.handle.net/10138/312138
dc.description.abstract Aim: The horse joint, due to its similarity with the human joint, is the ultimate model for translational articular cartilage repair studies. This study was designed to determine the critical size of cartilage defects in the equine carpus and serve as a benchmark for the evaluation of new cartilage treatment options. Material and Methods: Circular full-thickness cartilage defects with a diameter of 2, 4, and 8 mm were created in the left middle carpal joint and similar osteochondral (3.5 mm in depth) defects in the right middle carpal joint of 5 horses. Spontaneously formed repair tissue was examined macroscopically, with MR and mu CT imaging, polarized light microscopy, standard histology, and immunohistochemistry at 12 months. Results: Filling of 2 mm chondral defects was good (77.8 +/- 8.5%), but proteoglycan depletion was evident in Safranin-O staining and gadolinium-enhanced MRI (T-1Gd). Larger chondral defects showed poor filling (50.6 +/- 2.7% in 4 mm and 31.9 +/- 7.3% in 8 mm defects). Lesion filling in 2, 4, and 8 mm osteochondral defects was 82.3 +/- 3.0%, 68.0 +/- 4.6% and 70.8 +/- 15.4%, respectively. Type II collagen staining was seen in 9/15 osteochondral defects but only in 1/15 chondral defects. Subchondral bone pathologies were evident in 14/15 osteochondral samples but only in 5/15 chondral samples. Although osteochondral lesions showed better neotissue quality than chondral lesions, the overall repair was deemed unsatisfactory because of the subchondral bone pathologies. Conclusion: We recommend classifying 4 mm as critical osteochondral lesion size and 2 mm as critical chondral lesion size for cartilage repair research in the equine carpal joint model. en
dc.format.extent 12
dc.language.iso eng
dc.relation.ispartof Connective Tissue Research
dc.rights unspecified
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Animal model
dc.subject cartilage repair
dc.subject critical-sized defect
dc.subject preclinical research
dc.subject spontaneous repair
dc.subject OSTEOCHONDRAL DEFECTS
dc.subject ANIMAL-MODELS
dc.subject REPAIR
dc.subject BONE
dc.subject TOMOGRAPHY
dc.subject TISSUE
dc.subject HORSE
dc.subject 1182 Biochemistry, cell and molecular biology
dc.subject 3126 Surgery, anesthesiology, intensive care, radiology
dc.title Critical-sized cartilage defects in the equine carpus en
dc.type Article
dc.contributor.organization I kirurgian klinikka (Töölö)
dc.contributor.organization Clinicum
dc.contributor.organization Department of Surgery
dc.contributor.organization HUS Musculoskeletal and Plastic Surgery
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1080/03008207.2018.1455670
dc.relation.issn 0300-8207
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

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