Single gene enables plant pathogenic Pectobacterium to overcome host-specific chemical defence

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http://hdl.handle.net/10138/312342

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van den Bosch , T J M , Niemi , O & Welte , C U 2020 , ' Single gene enables plant pathogenic Pectobacterium to overcome host-specific chemical defence ' , Molecular Plant Pathology , vol. 21 , no. 3 , pp. 349-359 . https://doi.org/10.1111/mpp.12900

Title: Single gene enables plant pathogenic Pectobacterium to overcome host-specific chemical defence
Author: van den Bosch, Tijs J. M.; Niemi, Outi; Welte, Cornelia U.
Contributor: University of Helsinki, Faculty of Biological and Environmental Sciences
Date: 2020-03
Language: eng
Number of pages: 11
Belongs to series: Molecular Plant Pathology
ISSN: 1464-6722
URI: http://hdl.handle.net/10138/312342
Abstract: Plants of the Brassicales order, including Arabidopsis and many common vegetables, produce toxic isothiocyanates to defend themselves against pathogens. Despite this defence, plant pathogenic microorganisms like Pectobacterium cause large yield losses in fields and during storage of crops. The bacterial gene saxA was previously found to encode isothiocyanate hydrolase that degrades isothiocyanates in vitro. Here we demonstrate in planta that saxA is a virulence factor that can overcome the chemical defence system of Brassicales plants. Analysis of the distribution of saxA genes in Pectobacterium suggests that saxA from three different phylogenetic origins are present within this genus. Deletion of saxA genes representing two of the most common classes from P. odoriferum and P. versatile resulted in significantly reduced virulence on Arabidopsis thaliana and Brassica oleracea. Furthermore, expressing saxA from a plasmid in a potato-specific P. parmentieri strain that does not naturally harbour this gene significantly increased the ability of the strain to macerate Arabidopsis. These findings suggest that a single gene may have a significant role in defining the host range of a plant pathogen.
Subject: 11831 Plant biology
4111 Agronomy
Arabidopsis
isothiocyanate hydrolase
host-specific virulence
Pectobacterium
soft rot
SOFT-ROT
ARABIDOPSIS
IDENTIFICATION
HYDROLYSIS
RESISTANCE
DICKEYA
SAXA
ROOT
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