The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum

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http://hdl.handle.net/10138/312373

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Linares , N C , Di Falco , M , Benoit-Gelber , I , Gruben , B S , Peng , M , Tsang , A , Mäkelä , M R & de Vries , R P 2019 , ' The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum ' , New Biotechnology , vol. 51 , pp. 57-66 . https://doi.org/10.1016/j.nbt.2019.02.005

Title: The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum
Author: Linares, Nancy Coconi; Di Falco, Marcos; Benoit-Gelber, Isabelle; Gruben, Birgit S.; Peng, Mao; Tsang, Adrian; Mäkelä, Miia R.; de Vries, Ronald P.
Contributor: University of Helsinki, Helsinki Institute of Sustainability Science (HELSUS)
Date: 2019-07-25
Language: eng
Number of pages: 10
Belongs to series: New Biotechnology
ISSN: 1871-6784
URI: http://hdl.handle.net/10138/312373
Abstract: Guar gum consists mainly of galactomannan and constitutes the endosperm of guar seeds that acts as a reserve polysaccharide for germination. Due to its molecular structure and physical properties, this biopolymer has been considered as one of the most important and widely used gums in industry. However, for many of these applications this (hemi-) cellulosic structure needs to be modified or (partially) depolymerized in order to customize and improve its physicochemical properties. In this study, transcriptome, exoproteome and enzyme activity analyses were employed to decipher the complete enzymatic arsenal for guar gum depolymerization by Aspergillus niger. This multi-omic analysis revealed a set of 46 genes encoding carbohydrate-active enzymes (CAZymes) responding to the presence of guar gum, including CAZymes not only with preferred activity towards galactomannan, but also towards (arabino-) xylan, cellulose, starch and pectin, likely due to trace components in guar gum. This demonstrates that the purity of substrates has a strong effect on the resulting enzyme mixture produced by A. niger and probably by other fungi as well, which has significant implications for the commercial production of fungal enzyme cocktails.
Subject: Guar gum
Aspergillus niger
CAZymes
Plant biomass degradation
Transcriptome
Exoproteome
TRANSCRIPTIONAL ACTIVATOR XLNR
MANNAN UTILIZATION SYSTEM
ALPHA-GALACTOSIDASE
EXPRESSION
GENES
HYDROLYSIS
NORMALIZATION
REGULATOR
RELEASE
XYLOSE
1182 Biochemistry, cell and molecular biology
1183 Plant biology, microbiology, virology
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