The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum

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dc.contributor.author Linares, Nancy Coconi
dc.contributor.author Di Falco, Marcos
dc.contributor.author Benoit-Gelber, Isabelle
dc.contributor.author Gruben, Birgit S.
dc.contributor.author Peng, Mao
dc.contributor.author Tsang, Adrian
dc.contributor.author Mäkelä, Miia R.
dc.contributor.author de Vries, Ronald P.
dc.date.accessioned 2020-02-27T03:11:28Z
dc.date.available 2021-12-18T03:45:59Z
dc.date.issued 2019-07-25
dc.identifier.citation Linares , N C , Di Falco , M , Benoit-Gelber , I , Gruben , B S , Peng , M , Tsang , A , Mäkelä , M R & de Vries , R P 2019 , ' The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum ' , New Biotechnology , vol. 51 , pp. 57-66 . https://doi.org/10.1016/j.nbt.2019.02.005
dc.identifier.other PURE: 126373121
dc.identifier.other PURE UUID: dd76fad7-8843-4adc-86f1-5a6f0f12a1c4
dc.identifier.other WOS: 000461362500008
dc.identifier.other Scopus: 85061999529
dc.identifier.other ORCID: /0000-0003-0771-2329/work/68618531
dc.identifier.uri http://hdl.handle.net/10138/312373
dc.description.abstract Guar gum consists mainly of galactomannan and constitutes the endosperm of guar seeds that acts as a reserve polysaccharide for germination. Due to its molecular structure and physical properties, this biopolymer has been considered as one of the most important and widely used gums in industry. However, for many of these applications this (hemi-) cellulosic structure needs to be modified or (partially) depolymerized in order to customize and improve its physicochemical properties. In this study, transcriptome, exoproteome and enzyme activity analyses were employed to decipher the complete enzymatic arsenal for guar gum depolymerization by Aspergillus niger. This multi-omic analysis revealed a set of 46 genes encoding carbohydrate-active enzymes (CAZymes) responding to the presence of guar gum, including CAZymes not only with preferred activity towards galactomannan, but also towards (arabino-) xylan, cellulose, starch and pectin, likely due to trace components in guar gum. This demonstrates that the purity of substrates has a strong effect on the resulting enzyme mixture produced by A. niger and probably by other fungi as well, which has significant implications for the commercial production of fungal enzyme cocktails. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof New Biotechnology
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Guar gum
dc.subject Aspergillus niger
dc.subject CAZymes
dc.subject Plant biomass degradation
dc.subject Transcriptome
dc.subject Exoproteome
dc.subject TRANSCRIPTIONAL ACTIVATOR XLNR
dc.subject MANNAN UTILIZATION SYSTEM
dc.subject ALPHA-GALACTOSIDASE
dc.subject EXPRESSION
dc.subject GENES
dc.subject HYDROLYSIS
dc.subject NORMALIZATION
dc.subject REGULATOR
dc.subject RELEASE
dc.subject XYLOSE
dc.subject 1182 Biochemistry, cell and molecular biology
dc.subject 1183 Plant biology, microbiology, virology
dc.title The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum en
dc.type Article
dc.contributor.organization Helsinki Institute of Sustainability Science (HELSUS)
dc.contributor.organization Department of Microbiology
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.nbt.2019.02.005
dc.relation.issn 1871-6784
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

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