Immobilization of natural lipid biomembranes and their interactions with choline carboxylates : A Nanoplasmonic sensing study

Show full item record



Permalink

http://hdl.handle.net/10138/312376

Citation

Dusa , F , Chen , W , Witos , J , Rantamäki , A , King , A , Sklavounos , E , Roth , M & Wiedmer , S 2020 , ' Immobilization of natural lipid biomembranes and their interactions with choline carboxylates : A Nanoplasmonic sensing study ' , Biochimica et Biophysica Acta. Biomembranes , vol. 1862 , no. 2 , 183115 . https://doi.org/10.1016/j.bbamem.2019.183115

Title: Immobilization of natural lipid biomembranes and their interactions with choline carboxylates : A Nanoplasmonic sensing study
Author: Dusa, Filip; Chen, Wen; Witos, Joanna; Rantamäki, Antti; King, Alistair; Sklavounos, Evangelos; Roth, Michal; Wiedmer, Susanne
Contributor: University of Helsinki, Department of Chemistry
University of Helsinki, Department of Chemistry
University of Helsinki, Department of Chemistry
University of Helsinki, Department of Chemistry
University of Helsinki, Department of Chemistry
Date: 2020-02-01
Language: eng
Number of pages: 13
Belongs to series: Biochimica et Biophysica Acta. Biomembranes
ISSN: 0005-2736
URI: http://hdl.handle.net/10138/312376
Abstract: The cell membrane is mainly composed of lipid bilayers with inserted proteins and carbohydrates. Lipid bilayers made of purified or synthetic lipids are widely used for estimating the effect of target compounds on cell membranes. However, the composition of such biomimetic membranes is much simpler than the composition of biological membranes. Interactions between compounds and simple composition biomimetic membranes might not demonstrate the effect of target compounds as precisely as membranes with compositions close to real organisms. Therefore, the aim of our study is to construct biomimetic membrane closely mimicking the state of natural membranes. Liposomes were prepared from lipids extracted from L-alpha-phosphatidylcholine, Escherichia coli, yeast (Saccharomyces cerevisiae) and bovine liver cells through agitation and sonication. They were immobilized onto silicon dioxide (SiO2) sensor surfaces using N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid buffer with calcium chloride. The biomimetic membranes were successfully immobilized onto the SiO2 sensor surface and detected by nanoplasmonic sensing. The immobilized membranes were exposed to choline carboxylates. The membrane disruption effect was, as expected, more pronounced with increasing carbohydrate chain length of the carboxylates. The results correlated with the toxicity values determined using Vibrio fischeri bacteria. The yeast extracted lipid membranes had the strongest response to introduction of choline laurate while the bovine liver lipid extracted liposomes were the most sensitive towards the shorter choline carboxylates. This implies that the composition of the cell membrane plays a crucial role upon interaction with choline carboxylates, and underlines the necessity of testing membrane systems of different origin to obtain an overall image of such interactions.
Subject: 116 Chemical sciences
Natural lipid biomembrane
Choline carboxylate
Nanoplasmonic sensing
Interaction
Ionic liquid
Immobilization
IONIC LIQUIDS
LIPOSOMES
VESICLES
DEFORMATION
CAPILLARIES
MEMBRANES
TOXICITY
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
20191011_Manuscript_R1_FINAL.pdf 326.6Kb PDF View/Open

This item appears in the following Collection(s)

Show full item record