Immobilization of natural lipid biomembranes and their interactions with choline carboxylates : A Nanoplasmonic sensing study

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dc.contributor.author Dusa, Filip
dc.contributor.author Chen, Wen
dc.contributor.author Witos, Joanna
dc.contributor.author Rantamäki, Antti
dc.contributor.author King, Alistair
dc.contributor.author Sklavounos, Evangelos
dc.contributor.author Roth, Michal
dc.contributor.author Wiedmer, Susanne
dc.date.accessioned 2020-02-27T06:52:02Z
dc.date.available 2020-02-27T06:52:02Z
dc.date.issued 2020-02-01
dc.identifier.citation Dusa , F , Chen , W , Witos , J , Rantamäki , A , King , A , Sklavounos , E , Roth , M & Wiedmer , S 2020 , ' Immobilization of natural lipid biomembranes and their interactions with choline carboxylates : A Nanoplasmonic sensing study ' , Biochimica et Biophysica Acta. Biomembranes , vol. 1862 , no. 2 , 183115 . https://doi.org/10.1016/j.bbamem.2019.183115
dc.identifier.other PURE: 127898196
dc.identifier.other PURE UUID: 9c8f263c-72f4-41c6-814e-092d8f71364b
dc.identifier.other WOS: 000509632200047
dc.identifier.other ORCID: /0000-0003-3142-9259/work/70943242
dc.identifier.other ORCID: /0000-0002-3097-6165/work/70943671
dc.identifier.other ORCID: /0000-0001-8490-6069/work/70952623
dc.identifier.uri http://hdl.handle.net/10138/312376
dc.description.abstract The cell membrane is mainly composed of lipid bilayers with inserted proteins and carbohydrates. Lipid bilayers made of purified or synthetic lipids are widely used for estimating the effect of target compounds on cell membranes. However, the composition of such biomimetic membranes is much simpler than the composition of biological membranes. Interactions between compounds and simple composition biomimetic membranes might not demonstrate the effect of target compounds as precisely as membranes with compositions close to real organisms. Therefore, the aim of our study is to construct biomimetic membrane closely mimicking the state of natural membranes. Liposomes were prepared from lipids extracted from L-alpha-phosphatidylcholine, Escherichia coli, yeast (Saccharomyces cerevisiae) and bovine liver cells through agitation and sonication. They were immobilized onto silicon dioxide (SiO2) sensor surfaces using N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid buffer with calcium chloride. The biomimetic membranes were successfully immobilized onto the SiO2 sensor surface and detected by nanoplasmonic sensing. The immobilized membranes were exposed to choline carboxylates. The membrane disruption effect was, as expected, more pronounced with increasing carbohydrate chain length of the carboxylates. The results correlated with the toxicity values determined using Vibrio fischeri bacteria. The yeast extracted lipid membranes had the strongest response to introduction of choline laurate while the bovine liver lipid extracted liposomes were the most sensitive towards the shorter choline carboxylates. This implies that the composition of the cell membrane plays a crucial role upon interaction with choline carboxylates, and underlines the necessity of testing membrane systems of different origin to obtain an overall image of such interactions. en
dc.format.extent 13
dc.language.iso eng
dc.relation.ispartof Biochimica et Biophysica Acta. Biomembranes
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 116 Chemical sciences
dc.subject Natural lipid biomembrane
dc.subject Choline carboxylate
dc.subject Nanoplasmonic sensing
dc.subject Interaction
dc.subject Ionic liquid
dc.subject Immobilization
dc.subject IONIC LIQUIDS
dc.subject LIPOSOMES
dc.subject VESICLES
dc.subject DEFORMATION
dc.subject CAPILLARIES
dc.subject MEMBRANES
dc.subject TOXICITY
dc.title Immobilization of natural lipid biomembranes and their interactions with choline carboxylates : A Nanoplasmonic sensing study en
dc.type Article
dc.contributor.organization Department of Chemistry
dc.contributor.organization HUS Head and Neck Center
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.bbamem.2019.183115
dc.relation.issn 0005-2736
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion

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