LFRET, a novel rapid assay for anti-tissue transglutaminase antibody detection

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dc.contributor University of Helsinki, Medicum en
dc.contributor University of Helsinki, HUSLAB en
dc.contributor University of Helsinki, Helsinki One Health (HOH) en
dc.contributor University of Helsinki, Viral Zoonosis Research Unit en
dc.contributor University of Helsinki, HUS Children and Adolescents en
dc.contributor University of Helsinki, HUSLAB en
dc.contributor.author Rusanen, Juuso
dc.contributor.author Toivonen, Anne
dc.contributor.author Hepojoki, Jussi
dc.contributor.author Hepojoki, Satu
dc.contributor.author Arikoski, Pekka
dc.contributor.author Heikkinen, Markku
dc.contributor.author Vaarala, Outi
dc.contributor.author Ilonen, Jorma
dc.contributor.author Hedman, Klaus
dc.date.accessioned 2020-03-02T06:56:01Z
dc.date.available 2020-03-02T06:56:01Z
dc.date.issued 2019-11-29
dc.identifier.citation Rusanen , J , Toivonen , A , Hepojoki , J , Hepojoki , S , Arikoski , P , Heikkinen , M , Vaarala , O , Ilonen , J & Hedman , K 2019 , ' LFRET, a novel rapid assay for anti-tissue transglutaminase antibody detection ' , PLoS One , vol. 14 , no. 11 , e0225851 . https://doi.org/10.1371/journal.pone.0225851 en
dc.identifier.issn 1932-6203
dc.identifier.other PURE: 130919499
dc.identifier.other PURE UUID: ecd30425-6362-40e4-bd29-7c620678b712
dc.identifier.other ORCID: /0000-0003-1779-7960/work/70941758
dc.identifier.other ORCID: /0000-0001-5699-214X/work/70944849
dc.identifier.other ORCID: /0000-0002-3954-5118/work/70950554
dc.identifier.other WOS: 000533892300041
dc.identifier.uri http://hdl.handle.net/10138/312586
dc.description.abstract The diagnosis of celiac disease (CD) is currently based on serology and intestinal biopsy, with detection of anti-tissue transglutaminase (tTG) IgA antibodies recommended as the first-line test. Emphasizing the increasing importance of serological testing, new guidelines and evidence suggest basing the diagnosis solely on serology without confirmatory biopsy. Enzyme immunoassays (EIAs) are the established approach for anti-tTG antibody detection, with the existing point-of-care (POC) tests lacking sensitivity and/or specificity. Improved POC methods could help reduce the underdiagnosis and diagnostic delay of CD. We have previously developed rapid homogenous immunoassays based on time-resolved Förster resonance energy transfer (TR-FRET), and demonstrated their suitability in serodiagnostics with hanta- and Zika virus infections as models. In this study, we set out to establish a protein L -based TR-FRET assay (LFRET) for the detection of anti-tTG antibodies. We studied 74 patients with biopsy-confirmed CD and 70 healthy controls, with 1) the new tTG-LFRET assay, and for reference 2) a well-established EIA and 3) an existing commercial POC test. IgG depletion was employed to differentiate between anti-tTG IgA and IgG positivity. The sensitivity and specificity of the first-generation tTG-LFRET POC assay in detection of CD were 87.8% and 94.3%, respectively, in line with those of the reference POC test. The sensitivity and specificity of EIA were 95.9% and 91.9%, respectively. This study demonstrates the applicability of LFRET to serological diagnosis of autoimmune diseases in general and of CD in particular. en
dc.format.extent 12
dc.language.iso eng
dc.relation.ispartof PLoS One
dc.rights en
dc.subject 3111 Biomedicine en
dc.subject 318 Medical biotechnology en
dc.subject 11832 Microbiology and virology en
dc.title LFRET, a novel rapid assay for anti-tissue transglutaminase antibody detection en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1371/journal.pone.0225851
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
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