Beneficial effects of running and milk protein supplements on Sirtuins and risk factors of metabolic disorders in rats with low aerobic capacity

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dc.contributor University of Helsinki, Department of Oral and Maxillofacial Diseases en
dc.contributor.author Lensu, S.
dc.contributor.author Pekkala, S.P.
dc.contributor.author Mäkinen, A.
dc.contributor.author Karstunen, N.
dc.contributor.author Turpeinen, A.T.
dc.contributor.author Hulmi, J.J.
dc.contributor.author Silvennoinen, M.M.
dc.contributor.author Ma, H.
dc.contributor.author Kujala, U.M.
dc.contributor.author Karvinen, S.
dc.contributor.author Koch, L.G.
dc.contributor.author Britton, S.L.
dc.contributor.author Kainulainen, H.
dc.date.accessioned 2020-03-03T06:15:01Z
dc.date.available 2020-03-03T06:15:01Z
dc.date.issued 2019-10-10
dc.identifier.citation Lensu , S , Pekkala , S P , Mäkinen , A , Karstunen , N , Turpeinen , A T , Hulmi , J J , Silvennoinen , M M , Ma , H , Kujala , U M , Karvinen , S , Koch , L G , Britton , S L & Kainulainen , H 2019 , ' Beneficial effects of running and milk protein supplements on Sirtuins and risk factors of metabolic disorders in rats with low aerobic capacity ' , Metabolism open , vol. 4 , 100019 . https://doi.org/10.1016/j.metop.2019.100019 en
dc.identifier.issn 2589-9368
dc.identifier.other PURE: 127742586
dc.identifier.other PURE UUID: 47f1ab0f-d626-41c2-ab90-3c957e354a38
dc.identifier.other RIS: urn:3ABF36BF318BBF62849D74892E3556C3
dc.identifier.uri http://hdl.handle.net/10138/312690
dc.description.abstract Background Physical activity and dietary intake of dairy products are associated with improved metabolic health. Dairy products are rich with branched chain amino acids that are essential for energy production. To gain insight into the mechanisms underlying the benefit of the sub-chronic effects of running and intake of milk protein supplements, we studied Low Capacity Runner rats (LCR), a rodent exercise model with risk for metabolic disorders. We especially focused on the role of Sirtuins, energy level dependent proteins that affect many cellular metabolic processes. Methods Forty-seven adult LCR female rats sedentary or running voluntarily in wheels were fed normal chow and given supplements of either whey or milk protein drink (PD)-supplemented water, or water only for 21 weeks. Physiological responses were measured in vivo. Blood lipids were determined from serum. Mitochondrial markers and Sirtuins (Sirt1-7) including downstream targets were measured in plantaris muscle by western blotting. Results For the first 10 weeks whey-drinking rats ran about 50% less compared to other groups; still, in all runners glucose tolerance improved and triglycerides decreased. Generally, running induced a ∼six-fold increase in running capacity and a ∼8% decrease in % body fat. Together with running, protein supplements increased the relative lean mass of the total body weight by ∼11%. In comparison with sedentary controls, running and whey increased HDL (21%) and whey, with or without running, lowered LDL (−34%). Running increased mitochondrial biogenesis and Sirtuins 3 and 4. When combined with exercise, both whey and milk protein drink induced about a 4-fold increase in Sirt3, compared to runners drinking water only, and about a 2-fold increase compared to the respective sedentary group. Protein supplements, with or without running, enhanced the phosphorylation level of the acetyl-coA-carboxylase, suggesting increased fat oxidation. Both supplemented diets increased Sirt5 and Sirt7 without an additional effect from exercise. Running diminished and PD supplement increased Sirt6. Conclusion We demonstrate in rats new sub-chronic effects of milk proteins on metabolism that involve Sirtuins and their downstream targets in skeletal muscle. The results show that running and milk proteins act on reducing the risk factors of metabolic disorders and suggest that the underlying mechanisms may involve Sirtuins. Notably, we found that milk protein supplements have some favorable effects on metabolism even without running. en
dc.format.extent 13
dc.language.iso eng
dc.relation.ispartof Metabolism open
dc.rights en
dc.subject Low capacity running rat (LCR) en
dc.subject Metabolism en
dc.subject Muscle en
dc.subject Running en
dc.subject Sirtuins en
dc.subject Whey en
dc.subject 315 Sport and fitness sciences en
dc.title Beneficial effects of running and milk protein supplements on Sirtuins and risk factors of metabolic disorders in rats with low aerobic capacity en
dc.type Article
dc.description.version Peer reviewed
dc.identifier.doi https://doi.org/10.1016/j.metop.2019.100019
dc.type.uri info:eu-repo/semantics/other
dc.type.uri info:eu-repo/semantics/publishedVersion
dc.contributor.pbl
dc.contributor.pbl
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