Neuronal activation stimulates cytomegalovirus promoter-driven transgene expression

Show full item record



Bäck , S , Dossat , A , Parkkinen , I , Koivula , P , Airavaara , M , Richie , C T , Chen , Y-H , Wang , Y & Harvey , B K 2019 , ' Neuronal activation stimulates cytomegalovirus promoter-driven transgene expression ' , Molecular therapy-Methods & clinical development , vol. 14 , pp. 180-188 .

Title: Neuronal activation stimulates cytomegalovirus promoter-driven transgene expression
Author: Bäck, Susanne; Dossat, Amanda; Parkkinen, Ilmari; Koivula, Pyry; Airavaara, Mikko; Richie, Christopher T.; Chen, Yun-Hsiang; Wang, Yun; Harvey, Brandon K.
Contributor organization: Institute of Biotechnology
Helsinki Institute of Life Science HiLIFE
Neuroscience Center
Date: 2019-09-13
Language: eng
Number of pages: 9
Belongs to series: Molecular therapy-Methods & clinical development
ISSN: 2329-0501
Abstract: The cytomegalovirus (CMV) immediate early promoter has been extensively developed and exploited for transgene expression in vitro and in vivo, including human clinical trials. The CMV promoter has long been considered a stable, constitutive and ubiquitous promoter for transgene expression. Using two different CMV-based promoters, we found an increase in CMV-driven transgene expression in the rodent brain and in primary neuronal cultures in response to methamphetamine, glutamate, kainic acid, and activation of G-protein coupled receptor signaling using designer receptors exclusively activated by designer drugs (DREADDs). In contrast, promoters derived from human synapsin 1 (hSyn1) gene or elongation factor 1a (EF1a) did not exhibit altered transgene expression in response to the same neuronal stimulation. Overall, our results suggest that the long standing assertion that the CMV promoter confers constitutive expression in neurons should be reevaluated and future studies should evaluate the activity of the CMV promoter in a given application.
Subject: 3112 Neurosciences
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
1_s2.0_S2329050119300671_main.pdf 2.304Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record