Role of Pneumococcal NanA Neuraminidase Activity in Peripheral Blood

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Syed , S , Hakala , P , Singh , A K , Lapatto , H A K , King , S J , Meri , S , Jokiranta , T S & Haapasalo , K 2019 , ' Role of Pneumococcal NanA Neuraminidase Activity in Peripheral Blood ' , Frontiers in Cellular and Infection Microbiology , vol. 9 , 218 . https://doi.org/10.3389/fcimb.2019.00218

Title: Role of Pneumococcal NanA Neuraminidase Activity in Peripheral Blood
Author: Syed, Shahan; Hakala, Pipsa; Singh, Anirudh K.; Lapatto, Helena A. K.; King, Samantha J.; Meri, Seppo; Jokiranta, T. Sakari; Haapasalo, Karita
Contributor organization: Department of Bacteriology and Immunology
University of Helsinki
Centre of Excellence in Stem Cell Metabolism
Medicum
HUSLAB
Seppo Meri / Principal Investigator
Date: 2019-06-26
Language: eng
Number of pages: 11
Belongs to series: Frontiers in Cellular and Infection Microbiology
ISSN: 2235-2988
DOI: https://doi.org/10.3389/fcimb.2019.00218
URI: http://hdl.handle.net/10138/312801
Abstract: The most frequent form of hemolytic-uremic syndrome (HUS) is associated with infections caused by Shiga-like toxin-producing Enterohaemorrhagic Escherichia coli (STEC). In rarer cases HUS can be triggered by Streptococcus pneumoniae. While production of Shiga-like toxins explains STEC-HUS, the mechanisms of pneumococcal HUS are less well known. S. pneumoniae produces neuraminidases with activity against cell surface sialic acids that are critical for factor H-mediated complement regulation on cells and platelets. The aim of this study was to find out whether S. pneumoniae neuraminidase NanA could trigger complement activation and hemolysis in whole blood. We studied clinical S. pneumoniae isolates and two laboratory strains, a wild-type strain expressing NanA, and a NanA deletion mutant for their ability to remove sialic acids from various human cells and platelets. Red blood cell lysis and activation of complement was measured ex vivo by incubating whole blood with bacterial culture supernatants. We show here that NanA expressing S. pneumoniae strains and isolates are able to remove sialic acids from cells, and platelets. Removal of sialic acids by NanA increased complement activity in whole blood, while absence of NanA blocked complement triggering and hemolytic activity indicating that removal of sialic acids by NanA could potentially trigger pHUS.
Subject: 3111 Biomedicine
pneumococcus
sialidase
alternative pathway
pneumonia
inflammation
HEMOLYTIC-UREMIC SYNDROME
STREPTOCOCCUS-PNEUMONIAE
FACTOR-H
COMPLEMENT
BINDING
ACTIVATION
SIALIDASE
HEPARIN
SITE
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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