Soil exposure modifies the gut microbiota and supports immune tolerance in a mouse model

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http://hdl.handle.net/10138/312870

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Ottman , N , Ruokolainen , L , Suomalainen , A , Sinkko , H , Karisola , P , Lehtimaki , J , Lehto , M , Hanski , I , Alenius , H & Fyhrquist , N 2019 , ' Soil exposure modifies the gut microbiota and supports immune tolerance in a mouse model ' , Journal of Allergy and Clinical Immunology , vol. 143 , no. 3 , pp. 1198-1206.e12 . https://doi.org/10.1016/j.jaci.2018.06.024

Title: Soil exposure modifies the gut microbiota and supports immune tolerance in a mouse model
Author: Ottman, Noora; Ruokolainen, Lasse; Suomalainen, Alina; Sinkko, Hanna; Karisola, Piia; Lehtimaki, Jenni; Lehto, Maili; Hanski, Ilkka; Alenius, Harri; Fyhrquist, Nanna
Contributor: University of Helsinki, Biosciences
University of Helsinki, Helsinki Institute of Sustainability Science (HELSUS)
University of Helsinki, Department of Bacteriology and Immunology
University of Helsinki, Equine and Small Animal Medicine
University of Helsinki, HUMI - Human Microbiome Research
University of Helsinki, Centre of Excellence in Metapopulation Research
University of Helsinki, Biosciences
University of Helsinki, HUMI - Human Microbiome Research
University of Helsinki, HUMI - Human Microbiome Research
Date: 2019-03
Number of pages: 21
Belongs to series: Journal of Allergy and Clinical Immunology
ISSN: 0091-6749
URI: http://hdl.handle.net/10138/312870
Abstract: Background: Sufficient exposure to natural environments, in particular soil and its microbes, has been suggested to be protective against allergies. Objective: We aim at gaining more direct evidence of the environment-microbiota-health axis by studying the colonization of gut microbiota in mice after exposure to soil and by examining immune status in both a steady-state situation and during allergic inflammation. Methods: The gastrointestinal microbiota of mice housed on clean bedding or in contact with soil was analyzed by using 16S rRNA gene sequencing, and the data were combined with immune parameters measured in the gut mucosa, lung tissue, and serum samples. Results: We observed marked differences in the small intestinal and fecal microbiota composition between mice housed on clean bedding or in contact with soil, with a higher proportion of Bacteroidetes relative to Firmicutes in the soil group. The housing environment also influenced mouse intestinal gene expression, as shown by upregulated expression of the immunoregulatory markers IL-10, forkhead box P3, and cytotoxic T lymphocyte-associated protein 4 in the soil group. Importantly, using the murine asthma model, we found that exposure to soil polarizes the immune system toward T(H)1 and a higher level of anti-inflammatory signaling, alleviating T(H)2-ype allergic responses. The inflammatory status of the mice had a marked influence on the composition of the gut microbiota, suggesting bidirectional communication along the gut-lung axis. Conclusion: Our results provide evidence of the role of environmentally acquired microbes in alleviating against T(H)2-driven inflammation, which relates to allergic diseases.
Subject: Gut microbiota
biodiversity
immunity
living environment
gut-lung axis
mouse asthma model
SEGMENTED FILAMENTOUS BACTERIA
ENVIRONMENTAL BIODIVERSITY
HOUSE-DUST
ALLERGY
ASTHMA
INFLAMMATION
HEALTH
FARM
A20
SENSITIZATION
3111 Biomedicine
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