Soil exposure modifies the gut microbiota and supports immune tolerance in a mouse model

Show full item record



Ottman , N , Ruokolainen , L , Suomalainen , A , Sinkko , H , Karisola , P , Lehtimaki , J , Lehto , M , Hanski , I , Alenius , H & Fyhrquist , N 2019 , ' Soil exposure modifies the gut microbiota and supports immune tolerance in a mouse model ' , Journal of Allergy and Clinical Immunology , vol. 143 , no. 3 , pp. 1198-1206.e12 .

Title: Soil exposure modifies the gut microbiota and supports immune tolerance in a mouse model
Author: Ottman, Noora; Ruokolainen, Lasse; Suomalainen, Alina; Sinkko, Hanna; Karisola, Piia; Lehtimaki, Jenni; Lehto, Maili; Hanski, Ilkka; Alenius, Harri; Fyhrquist, Nanna
Contributor organization: Biosciences
Helsinki Institute of Sustainability Science (HELSUS)
Veijo Kaitala / Principal Investigator
Ilkka Hanski / Principal Investigator
Ecology and Evolutionary Biology
Organismal and Evolutionary Biology Research Programme
Department of Bacteriology and Immunology
Equine and Small Animal Medicine
HUMI - Human Microbiome Research
Centre of Excellence in Metapopulation Research
Date: 2019-03
Language: eng
Number of pages: 21
Belongs to series: Journal of Allergy and Clinical Immunology
ISSN: 0091-6749
Abstract: Background: Sufficient exposure to natural environments, in particular soil and its microbes, has been suggested to be protective against allergies. Objective: We aim at gaining more direct evidence of the environment-microbiota-health axis by studying the colonization of gut microbiota in mice after exposure to soil and by examining immune status in both a steady-state situation and during allergic inflammation. Methods: The gastrointestinal microbiota of mice housed on clean bedding or in contact with soil was analyzed by using 16S rRNA gene sequencing, and the data were combined with immune parameters measured in the gut mucosa, lung tissue, and serum samples. Results: We observed marked differences in the small intestinal and fecal microbiota composition between mice housed on clean bedding or in contact with soil, with a higher proportion of Bacteroidetes relative to Firmicutes in the soil group. The housing environment also influenced mouse intestinal gene expression, as shown by upregulated expression of the immunoregulatory markers IL-10, forkhead box P3, and cytotoxic T lymphocyte-associated protein 4 in the soil group. Importantly, using the murine asthma model, we found that exposure to soil polarizes the immune system toward T(H)1 and a higher level of anti-inflammatory signaling, alleviating T(H)2-ype allergic responses. The inflammatory status of the mice had a marked influence on the composition of the gut microbiota, suggesting bidirectional communication along the gut-lung axis. Conclusion: Our results provide evidence of the role of environmentally acquired microbes in alleviating against T(H)2-driven inflammation, which relates to allergic diseases.
Subject: Gut microbiota
living environment
gut-lung axis
mouse asthma model
3111 Biomedicine
Peer reviewed: Yes
Rights: cc_by_nc_nd
Usage restriction: openAccess
Self-archived version: acceptedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
Ottman_JACI_accepted_manuscript.pdf 2.820Mb PDF View/Open
Soil_exposure_m ... rance_in_a_mouse_model.pdf 2.895Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record