Salivary levels of MPO, MMP-8 and TIMP-1 are associated with gingival inflammation response patterns during experimental gingivitis

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Nascimento , G G , Baelum , V , Sorsa , T , Tervahartiala , T , Skottrup , P D & Lopez , R 2019 , ' Salivary levels of MPO, MMP-8 and TIMP-1 are associated with gingival inflammation response patterns during experimental gingivitis ' , Cytokine , vol. 115 , pp. 135-141 . https://doi.org/10.1016/j.cyto.2018.12.002

Title: Salivary levels of MPO, MMP-8 and TIMP-1 are associated with gingival inflammation response patterns during experimental gingivitis
Author: Nascimento, Gustavo G.; Baelum, Vibeke; Sorsa, Timo; Tervahartiala, Taina; Skottrup, Peter D.; Lopez, Rodrigo
Contributor organization: Department of Oral and Maxillofacial Diseases
Timo Sorsa / Principal Investigator
Suu- ja leukakirurgian yksikkö
University of Helsinki
Clinicum
HUS Head and Neck Center
Date: 2019-03
Language: eng
Number of pages: 7
Belongs to series: Cytokine
ISSN: 1043-4666
DOI: https://doi.org/10.1016/j.cyto.2018.12.002
URI: http://hdl.handle.net/10138/312875
Abstract: Aim: This study aimed to investigate the association between salivary levels of myeloperoxidase (MPO), neutrophil elastase (NE), soluble urokinase-type plasminogen activator receptor (suPAR), matrix metalloproteinase (MMP)-8 and tissue inhibitor of matrix metalloproteinases (TIMP)-1 and gingival inflammation development during an experimental gingivitis study. Methods: A three-week experimental gingivitis study was conducted. Clinical recordings of dental plaque biofilm (Modified Quigley Hein Plaque Index, TQHPI) and gingival inflammation (Modified Gingival Index, MGI) were made at specific time points for each of the 42 participants. Salivary levels of MPO, NE, suPAR, MMP-8 and TIMP-1 at the same time points were measured using distinct immunoassays. For data analysis growth curve modelling was employed to account for the time-varying outcome (MGI score) and the time-varying covariates (salivary marker levels, and TQHPI score). Analyses were stratified according to the MGI-score trajectory groups previously identified as 'fast', respectively 'slow' responders. Results: Overall, higher MGI scores were statistically significantly positively associated with higher levels of MPO, MMP-8 and TIMP-1. Stratified analysis according to inflammation development trajectory group revealed higher levels of salivary MPO, MMP-8 and MMP-8/TIMP-1 ratio among the 'fast' responders than among 'slow' responders. None of the investigated salivary protein markers was associated with a 'slow' inflammation development response. Conclusions: Salivary levels of MPO, MMP-8 and TIMP-1 were associated with the extent and severity of gingival inflammation. While the 'fast' gingival inflammation response was associated with increased levels of MPO, MMP-8 and MMP-8/TIMP-1 ratio, the 'slow' response was not associated with any of the salivary protein markers investigated in this study. Neutrophil activity seems to orchestrate a 'fast' gingival inflammatory response among participants previously primed to gingival inflammation.
Subject: Immunology
Innate immunity
Neutrophils
Gingival inflammation
Periodontal diseases
MATRIX METALLOPROTEINASE-8
FLUID
MYELOPEROXIDASE
MECHANISMS
HEALTH
SERUM
1182 Biochemistry, cell and molecular biology
3121 General medicine, internal medicine and other clinical medicine
313 Dentistry
Peer reviewed: Yes
Usage restriction: openAccess
Self-archived version: publishedVersion


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