Amphiphilic Polypeptides for VEGF siRNA Delivery into Retinal Epithelial Cells

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Osipova , O , Sharoyko , V , Zashikhina , N , Zakharova , N , Tennikova , T , Urtti , A & Korzhikova-Vlakh , E 2020 , ' Amphiphilic Polypeptides for VEGF siRNA Delivery into Retinal Epithelial Cells ' , Pharmaceutics , vol. 12 , no. 1 , 39 .

Title: Amphiphilic Polypeptides for VEGF siRNA Delivery into Retinal Epithelial Cells
Author: Osipova, Olga; Sharoyko, Vladimir; Zashikhina, Natalia; Zakharova, Natalya; Tennikova, Tatiana; Urtti, Arto; Korzhikova-Vlakh, Evgenia
Contributor organization: Drug Research Program
Drug Delivery
Drug Delivery Unit
Division of Pharmaceutical Biosciences
Date: 2020-01
Language: eng
Number of pages: 17
Belongs to series: Pharmaceutics
ISSN: 1999-4923
Abstract: Polyethyleneimine, poly-L-lysine, chitosan and some others cationic polymers have been thoroughly studied as nucleic acid delivery systems in gene therapy. However, the drug release from these systems proceeds at a very low rate due to extremely high binding between a carrier and gene material. To reduce these interactions and to enhance drug release, we developed a set of amphiphilic polypeptides containing positively and negatively charged amino acids as well as a hydrophobic one. The copolymers obtained were characterized by size-exclusion chromatography, static light scattering, HPLC amino acid analysis and (HNMR)-H-1 spectroscopy. All copolymers formed particles due to a self-assembly in aqueous media. Depending on polypeptide composition, the formation of particles with hydrodynamic diameters from 180 to 900 nm was observed. Stability of polymer particles, loading and release efficiency were carefully studied. Cellular uptake of the particles was efficient and their cytotoxicity was negligible. The application of polymer carriers, containing siRNA, to vascular endothelial growth factor (VEGF-A165) silencing of ARPE-19 cells was successful. The gene silencing was confirmed by suppression of both messenger RNA and protein expression.
Subject: amphiphilic polypeptides
siRNA delivery
gene silencing
317 Pharmacy
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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