An Oral Rinse Active Matrix Metalloproteinase-8 Point-of-Care Immunotest May Be Less Accurate in Patients with Crohn’s Disease

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dc.contributor.author Rautava, Jaana
dc.contributor.author Gürsoy, Ulvi K.
dc.contributor.author Kullström, Adrian
dc.contributor.author Könönen, Eija
dc.contributor.author Sorsa, Timo
dc.contributor.author Tervahartiala, Taina
dc.contributor.author Gürsoy, Mervi
dc.date.accessioned 2020-03-13T13:09:25Z
dc.date.available 2020-03-13T13:09:25Z
dc.date.issued 2020-03-04
dc.identifier.citation Rautava, J.; Gürsoy, U.K.; Kullström, A.; Könönen, E.; Sorsa, T.; Tervahartiala, T.; Gürsoy, M. An Oral Rinse Active Matrix Metalloproteinase-8 Point-of-Care Immunotest May Be Less Accurate in Patients with Crohn’s Disease. Biomolecules 2020, 10, 395.
dc.identifier.uri http://hdl.handle.net/10138/313296
dc.description.abstract The diagnostic accuracy of point-of-care (PoC) applications may be compromised in individuals with additional inflammatory conditions. This cross-sectional study examined the performance of a commercial oral rinse active matrix metalloproteinase-8 (aMMP-8) PoC immunotest in individuals with (<em>n</em> = 47) and without Crohn&rsquo;s disease (CD) (<em>n</em> = 41). Oral rinse collected from the participants was analyzed by the PoC immunotest. Molecular forms and fragments of salivary MMP-8 were detected by western immunoblotting. The sensitivity of the immunotest for periodontitis was 60.0% in the CD group and 90.0% in the control group. The respective specificity was 75.0% and 80.0%. In both groups, clinical diagnosis of periodontitis exhibited a significant association with the immunotest results, however, the odds ratio (OR) was more than ten-fold in controls (OR 54.3, 95% CI: 3.1&ndash;953, <em>p </em>= 0.006) in comparison to CD patients (OR 5.2, 95% CI: 1.3&ndash;21.6, <em>p </em>= 0.022). According to Western immunoblot results, the immunotest MMP-8 positivity was not related to elevated levels of molecular forms and fragments of MMP-8 in the CD group, as in the control group. The diagnostic accuracy of the aMMP-8 PoC oral rinse immunotest is reduced in CD patients, which may be related to lower levels or undetectable complexes.
dc.publisher Multidisciplinary Digital Publishing Institute
dc.title An Oral Rinse Active Matrix Metalloproteinase-8 Point-of-Care Immunotest May Be Less Accurate in Patients with Crohn’s Disease
dc.date.updated 2020-03-13T13:09:25Z
dc.type.uri http://purl.org/eprint/entityType/JournalArticle
dc.type.uri http://purl.org/eprint/entityType/Expression
dc.type.uri http://purl.org/eprint/entityType/Expression

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