A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales

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http://hdl.handle.net/10138/313343

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Matlashov , M E , Shcherbakova , D M , Alvelid , J , Baloban , M , Pennacchietti , F , Shemetov , A A , Testa , I & Verkhusha , V V 2020 , ' A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales ' , Nature Communications , vol. 11 , no. 1 , 239 . https://doi.org/10.1038/s41467-019-13897-6

Title: A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales
Author: Matlashov, Mikhail E.; Shcherbakova, Daria M.; Alvelid, Jonatan; Baloban, Mikhail; Pennacchietti, Francesca; Shemetov, Anton A.; Testa, Ilaria; Verkhusha, Vladislav V.
Contributor: University of Helsinki, Medicum
Date: 2020-01-13
Language: eng
Number of pages: 12
Belongs to series: Nature Communications
ISSN: 2041-1723
URI: http://hdl.handle.net/10138/313343
Abstract: Bright monomeric near-infrared (NIR) fluorescent proteins (FPs) are in high demand as protein tags for multicolor microscopy and in vivo imaging. Here we apply rational design to engineer a complete set of monomeric NIR FPs, which are the brightest genetically encoded NIR probes. We demonstrate that the enhanced miRFP series of NIR FPs, which combine high effective brightness in mammalian cells and monomeric state, perform well in both nanometer-scale imaging with diffraction unlimited stimulated emission depletion (STED) microscopy and centimeter-scale imaging in mice. In STED we achieve -40nm resolution in live cells. In living mice we detect -10(5) fluorescent cells in deep tissues. Using spectrally distinct monomeric NIR FP variants, we perform two-color live-cell STED microscopy and two-color imaging in vivo. Having emission peaks from 670nm to 720nm, the next generation of miRFPs should become versatile NIR probes for multiplexed imaging across spatial scales in different modalities.
Subject: MICROSCOPY
INSIGHTS
BRIGHT
LIGHT
KNOT
3111 Biomedicine
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