The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure

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dc.contributor.author Stsepetova, Jelena
dc.contributor.author Baranova, Juliana
dc.contributor.author Simm, Jaak
dc.contributor.author Parm, Ulle
dc.contributor.author Rööp, Tiiu
dc.contributor.author Sokmann, Sandra
dc.contributor.author Korrovits, Paul
dc.contributor.author Jaagura, Madis
dc.contributor.author Rosenstein, Karin
dc.contributor.author Salumets, Andres
dc.contributor.author Mandar, Reet
dc.date.accessioned 2020-03-16T14:44:01Z
dc.date.available 2020-03-16T14:44:01Z
dc.date.issued 2020-01-16
dc.identifier.citation Stsepetova , J , Baranova , J , Simm , J , Parm , U , Rööp , T , Sokmann , S , Korrovits , P , Jaagura , M , Rosenstein , K , Salumets , A & Mandar , R 2020 , ' The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure ' , Reproductive Biology and Endocrinology , vol. 18 , no. 1 , 3 . https://doi.org/10.1186/s12958-019-0562-z
dc.identifier.other PURE: 133713105
dc.identifier.other PURE UUID: d3a51b32-6778-434e-8c50-7206afcbbf12
dc.identifier.other WOS: 000513706400001
dc.identifier.uri http://hdl.handle.net/10138/313370
dc.description.abstract Background Only a few microbial studies have conducted in IVF (in vitro fertilization), showing the high-variety bacterial contamination of IVF culture media to cause damage to or even loss of cultured oocytes and embryos. We aimed to determine the prevalence and counts of bacteria in IVF samples, and to associate them with clinical outcome. Methods The studied samples from 50 infertile couples included: raw (n = 48), processed (n = 49) and incubated (n = 50) sperm samples, and IVF culture media (n = 50). The full microbiome was analyzed by 454 pyrosequencing and quantitative analysis by real-time quantitative PCR. Descriptive statistics, t-, Mann-Whitney tests and Spearman's correlation were used for comparison of studied groups. Results The study involved normozoospermic men. Normal vaginal microbiota was present in 72.0% of female partners, while intermediate microbiota and bacterial vaginosis were diagnosed in 12.0 and 16.0%, respectively. The decreasing bacterial loads were found in raw (35.5%), processed (12.0%) and sperm samples used for oocyte insemination (4.0%), and in 8.0% of IVF culture media. The most abundant genera of bacteria in native semen and IVF culture media were Lactobacillus, while in other samples Alphaproteobacteria prevailed. Staphylococcus sp. was found only in semen from patients with inflammation. Phylum Bacteroidetes was in negative correlation with sperm motility and Alphaproteobacteria with high-quality IVF embryos. Conclusion Our study demonstrates that IVF does not occur in a sterile environment. The prevalent bacteria include classes Bacilli in raw semen and IVF culture media, Clostridia in processed and Bacteroidia in sperm samples used for insemination. The presence of Staphylococcus sp. and Alphaproteobacteria associated with clinical outcomes, like sperm and embryo quality. en
dc.format.extent 13
dc.language.iso eng
dc.relation.ispartof Reproductive Biology and Endocrinology
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Bacteria
dc.subject Contamination
dc.subject In vitro fertilization (IVF)
dc.subject Infertility
dc.subject Sperm microbiota
dc.subject STIMULATING-HORMONE BINDING
dc.subject REAL-TIME PCR
dc.subject BACTERIAL VAGINOSIS
dc.subject RIBOSOMAL-RNA
dc.subject FOLLICULAR-FLUID
dc.subject IDENTIFICATION
dc.subject SEQUENCE
dc.subject CONTAMINATION
dc.subject INHIBITOR
dc.subject RECEPTOR
dc.subject 3121 General medicine, internal medicine and other clinical medicine
dc.title The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure en
dc.type Article
dc.contributor.organization HUS Gynecology and Obstetrics
dc.contributor.organization Clinicum
dc.contributor.organization Department of Obstetrics and Gynecology
dc.contributor.organization University of Helsinki
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1186/s12958-019-0562-z
dc.relation.issn 1477-7827
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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