Convergent evolution of [D-Leucine(1)] microcystin-LR in taxonomically disparate cyanobacteria

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Shishido , T K , Kaasalainen , U , Fewer , D P , Rouhiainen , L A O , Jokela , J , Wahlsten , M , Fiore , M F , Yunes , J S , Rikkinen , J & Sivonen , K 2013 , ' Convergent evolution of [D-Leucine(1)] microcystin-LR in taxonomically disparate cyanobacteria ' , BMC Evolutionary Biology , vol. 13 , 86 .

Title: Convergent evolution of [D-Leucine(1)] microcystin-LR in taxonomically disparate cyanobacteria
Author: Shishido, Tania Keiko; Kaasalainen, Ulla; Fewer, David Peter; Rouhiainen, Leo A O; Jokela, Jouni; Wahlsten, Matti; Fiore, Marli Fátima; Yunes, João Sarkis; Rikkinen, Jouko; Sivonen, Kaarina
Contributor organization: Department of Food and Nutrition
Plant Biology
Microbial Natural Products
Cyanobacteria research
Teachers' Academy
Date: 2013-04-19
Language: eng
Number of pages: 15
Belongs to series: BMC Evolutionary Biology
ISSN: 1471-2148
Abstract: BACKGROUND: Many important toxins and antibiotics are produced by non-ribosomal biosynthetic pathways. Microcystins are a chemically diverse family of potent peptide toxins and the end-products of a hybrid NRPS and PKS secondary metabolic pathway. They are produced by a variety of cyanobacteria and are responsible for the poisoning of humans as well as the deaths of wild and domestic animals around the world. The chemical diversity of the microcystin family is attributed to a number of genetic events that have resulted in the diversification of the pathway for microcystin assembly. RESULTS: Here, we show that independent evolutionary events affecting the substrate specificity of the microcystin biosynthetic pathway have resulted in convergence on a rare [D-Leu(1)] microcystin-LR chemical variant. We detected this rare microcystin variant from strains of the distantly related genera Microcystis, Nostoc, and Phormidium. Phylogenetic analysis performed using sequences of the catalytic domains within the mcy gene cluster demonstrated a clear recombination pattern in the adenylation domain phylogenetic tree. We found evidence for conversion of the gene encoding the McyA(2) adenylation domain in strains of the genera Nostoc and Phormidium. However, point mutations affecting the substrate-binding sequence motifs of the McyA(2) adenylation domain were associated with the change in substrate specificity in two strains of Microcystis. In addition to the main [D-Leu(1)] microcystin-LR variant, these two strains produced a new microcystin that was identified as [Met(1)] microcystin-LR. CONCLUSIONS: Phylogenetic analysis demonstrated that both point mutations and gene conversion result in functional mcy gene clusters that produce the same rare [D-Leu(1)] variant of microcystin in strains of the genera Microcystis, Nostoc, and Phormidium. Engineering pathways to produce recombinant non-ribosomal peptides could provide new natural products or increase the activity of known compounds. Our results suggest that the replacement of entire adenylation domains could be a more successful strategy to obtain higher specificity in the modification of the non-ribosomal peptides than point mutations.
Subject: 1183 Plant biology, microbiology, virology
1184 Genetics, developmental biology, physiology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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