Enzyme activity profiles produced on wood and straw by four fungi of different decay strategies

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Veloz Villavicencio , E E , Mali , T , Mattila , H & Lundell , T 2020 , ' Enzyme activity profiles produced on wood and straw by four fungi of different decay strategies ' , Microorganisms , vol. 8 , no. 1 , 73 . https://doi.org/10.3390/microorganisms8010073

Title: Enzyme activity profiles produced on wood and straw by four fungi of different decay strategies
Author: Veloz Villavicencio, Eliana Estefanía; Mali, Tuulia; Mattila, Hans; Lundell, Taina
Contributor: University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
University of Helsinki, Department of Microbiology
Date: 2020-01-02
Language: eng
Number of pages: 18
Belongs to series: Microorganisms
ISSN: 2076-2607
URI: http://hdl.handle.net/10138/313784
Abstract: Four well-studied saprotrophic Basidiomycota Agaricomycetes species with different decay strategies were cultivated on solid lignocellulose substrates to compare their extracellular decomposing carbohydrate-active and lignin-attacking enzyme production profiles. Two Polyporales species, the white rot fungus Phlebia radiata and brown rot fungus Fomitopsis pinicola, as well as one Agaricales species, the intermediate “grey” rot fungus Schizophyllum commune, were cultivated on birch wood pieces for 12 weeks, whereas the second Agaricales species, the litter-decomposing fungus Coprinopsis cinerea was cultivated on barley straw for 6 weeks under laboratory conditions. During 3 months of growth on birch wood, only the white rot fungus P. radiata produced high laccase and MnP activities. The brown rot fungus F. pinicola demonstrated notable production of xylanase activity up to 43 nkat/mL on birch wood, together with moderate β-glucosidase and endoglucanase cellulolytic activities. The intermediate rot fungus S. commune was the strongest producer of β-glucosidase with activities up to 54 nkat/mL, and a notable producer of xylanase activity, even up to 620 nkat/mL, on birch wood. Low lignin-attacking but moderate activities against cellulose and hemicellulose were observed with the litter-decomposer C. cinerea on barley straw. Overall, our results imply that plant cell wall decomposition ability of taxonomically and ecologically divergent fungi is in line with their enzymatic decay strategy, which is fundamental in understanding their physiology and potential for biotechnological applications.
Subject: 414 Agricultural biotechnology
Wood biodegradation
Waste biomass
11831 Plant biology
Basidiomycota
wood decay fungi
lignocellulose
biodegradation
carbohydrate active enzymes
laccase
manganese peroxidase
enzyme activity assays
organic acids
oxalic acid
COPRINOPSIS-CINEREA
GENOME SEQUENCE
LIGNIN
DEGRADATION
MECHANISMS
EVOLUTION
fungal enzymes
enzyme activeity profile
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