Osmotrophic glucose and leucine assimilation and its impact on EPA and DHA content in algae

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dc.contributor.author Peltomaa, Elina T.
dc.contributor.author Taipale, Sami
dc.date.accessioned 2020-03-31T14:56:01Z
dc.date.available 2020-03-31T14:56:01Z
dc.date.issued 2020-01-03
dc.identifier.citation Peltomaa , E T & Taipale , S 2020 , ' Osmotrophic glucose and leucine assimilation and its impact on EPA and DHA content in algae ' , PeerJ , vol. 8 , 8363 . https://doi.org/10.7717/peerj.8363
dc.identifier.other PURE: 134587880
dc.identifier.other PURE UUID: d88a4a79-7c5f-4059-91a3-21612d73df99
dc.identifier.other WOS: 000505543000016
dc.identifier.other ORCID: /0000-0002-2858-8358/work/71511211
dc.identifier.uri http://hdl.handle.net/10138/313802
dc.description.abstract The uptake of dissolved organic compounds, that is, osmotrophy, has been shown to be an efficient nutritional strategy for algae. However, this mode of nutrition may affect the biochemical composition, for example, the fatty acid (FA) contents, of algal cells. This study focused on the osmotrophic assimilation of glucose and leucine by selected seven algal strains belonging to chlorophytes, chrysophytes, cryptophytes, dinoflagellates and euglenoids. Our laboratory experiments with stable isotope labeling showed that osmotrophy occurred in four of the selected seven strains. However, only three of these produced long chain omega-3 FAs eicosapentaenoic acid (EPA; 20:5 omega 3) and docosahexaenoic acid (DHA; 22:6 omega 3). High glucose content (5 mg L-1) affected negatively on the total FAs of Mallomonas kalinae and the total omega-3 FAs of Cryptomonas sp. Further, glucose assimilation explained 35% (negative effect) and leucine assimilation 48% (positive effect) of the variation of EPA, DHA and the FAs related to their synthesis in Cryptomonas sp. Moderate glucose concentration (2 mg L-1) was found to enhance the growth of Cryptomonas ozolinii, whereas low leucine (20 mu g L-1) enhanced the growth of M. kalinae. However, no systematic effect of osmotrophy on growth rates was detected. Our study shows that osmotrophic assimilation of algae is species and compound specific, and that the effects of the assimilated compounds on algal metabolism also varies depending on the species. en
dc.format.extent 16
dc.language.iso eng
dc.relation.ispartof PeerJ
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject Mixotrophy
dc.subject Omega-3 fatty acids
dc.subject Stable isotope labeling
dc.subject Cryptophytes
dc.subject FATTY-ACID
dc.subject GROWTH
dc.subject LAKE
dc.subject CARBON
dc.subject COMPETITORS
dc.subject METABOLISM
dc.subject SUCCESS
dc.subject QUALITY
dc.subject 1181 Ecology, evolutionary biology
dc.title Osmotrophic glucose and leucine assimilation and its impact on EPA and DHA content in algae en
dc.type Article
dc.contributor.organization Lammi Biological Station
dc.contributor.organization Faculty of Biological and Environmental Sciences
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.7717/peerj.8363
dc.relation.issn 2167-8359
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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