Label-Free Proteomics Approach Characterizes Plasma Protein Signature of Donor Brain Death

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dc.contributor.author Lukac, J.
dc.contributor.author Saraswat, Mayank
dc.contributor.author Holmström, E.
dc.contributor.author Dhaygude, K.
dc.contributor.author Joenväärä, Sakari
dc.contributor.author Krebs, R.
dc.contributor.author Nykänen, A.
dc.contributor.author Renkonen, R.
dc.contributor.author Lemström, K.
dc.date.accessioned 2020-04-01T01:53:39Z
dc.date.available 2021-12-17T22:45:42Z
dc.date.issued 2019-04
dc.identifier.citation Lukac , J , Saraswat , M , Holmström , E , Dhaygude , K , Joenväärä , S , Krebs , R , Nykänen , A , Renkonen , R & Lemström , K 2019 , ' Label-Free Proteomics Approach Characterizes Plasma Protein Signature of Donor Brain Death ' , Journal of Heart and Lung Transplantation , vol. 38 , no. 4, Supplement , pp. S159 . https://doi.org/10.1016/j.healun.2019.01.380
dc.identifier.other PURE: 123381538
dc.identifier.other PURE UUID: 760bf06e-cc84-46ba-be0a-ff81c9e86c93
dc.identifier.other RIS: urn:1FE706D468F756AE055EF3FB5441DCE4
dc.identifier.other ORCID: /0000-0003-4499-6180/work/70950379
dc.identifier.other ORCID: /0000-0001-6920-4093/work/70951561
dc.identifier.other WOS: 000461365101071
dc.identifier.other ORCID: /0000-0003-0418-8226/work/103594599
dc.identifier.uri http://hdl.handle.net/10138/313828
dc.description.abstract Purpose Despite recent advances in donation after circulatory death, transplants from brain-dead donors remain the sole source in heart transplantation (HTx) worldwide. Due to organ shortage, marginal donors are increasingly used and the utilization of transplants becomes markedly more challenging. They undergo invariably brain death that induces a systemic cytokine and catecholamine storm that lead to systemic inflammation, labile hemodynamics, and organ hypoperfusion. Together, these can damage the heart and aggravate later occurring graft injury, and ultimately, compromise graft function. However, the effect of donor brain death on allografts is not well understood. Methods In a separate prospective, blinded single-center trial, we collected donor plasma samples and relevant clinical patient data from 50 HTx brain-dead donors and as controls plasma samples from age- and gender-matched 23 healthy volunteers. Quantitative label-free proteomics in high definition MSE mode (HDMSE) was carried out on the samples. Various statistical analyses were performed on quantitative proteomics data to obtain the most reliably distinguishing proteins, which classify the donors vs controls. Results With two or more unique proteins per identification, 463 proteins were quantified in our pilot study. A complete separation between donors and controls based on a set of 278 proteins (p-value en
dc.format.extent 1
dc.language.iso eng
dc.relation.ispartof Journal of Heart and Lung Transplantation
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.title Label-Free Proteomics Approach Characterizes Plasma Protein Signature of Donor Brain Death en
dc.type Meeting Abstract
dc.contributor.organization HUSLAB
dc.contributor.organization Doctoral Programme in Biomedicine
dc.contributor.organization Clinicum
dc.contributor.organization Department of Pathology
dc.contributor.organization Doctoral Programme in Wildlife Biology
dc.contributor.organization Transplantation Laboratory
dc.contributor.organization HUS Heart and Lung Center
dc.contributor.organization Staff Services
dc.contributor.organization Department of Bacteriology and Immunology
dc.contributor.organization Risto Renkonen / Principal Investigator
dc.contributor.organization Infection Biology Research Program
dc.contributor.organization Department of Surgery
dc.contributor.organization University Management
dc.contributor.organization Doctoral Programme in Clinical Research
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.healun.2019.01.380
dc.relation.issn 1053-2498
dc.rights.accesslevel openAccess
dc.type.version acceptedVersion
dc.identifier.url http://www.sciencedirect.com/science/article/pii/S105324981930381X

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