Hypoxia is regulating enzymatic wood decomposition and intracellular carbohydrate metabolism in filamentous white rot fungus

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Mattila , H , Mäkinen , M & Lundell , T 2020 , ' Hypoxia is regulating enzymatic wood decomposition and intracellular carbohydrate metabolism in filamentous white rot fungus ' , Biotechnology for Biofuels , vol. 13 , no. 1 , 26 . https://doi.org/10.1186/s13068-020-01677-0

Title: Hypoxia is regulating enzymatic wood decomposition and intracellular carbohydrate metabolism in filamentous white rot fungus
Author: Mattila, Hans; Mäkinen, Mari; Lundell, Taina
Contributor organization: Department of Microbiology
Fungal Co-life, Omics and Ecophysiology Research Group
Helsinki Institute of Sustainability Science (HELSUS)
Date: 2020-02-24
Language: eng
Number of pages: 17
Belongs to series: Biotechnology for Biofuels
ISSN: 1754-6834
DOI: https://doi.org/10.1186/s13068-020-01677-0
URI: http://hdl.handle.net/10138/313974
Abstract: Background Fungal decomposition of wood is considered as a strictly aerobic process. However, recent findings on wood-decaying fungi to produce ethanol from various lignocelluloses under oxygen-depleted conditions lead us to question this. We designed gene expression study of the white rot fungus Phlebia radiata (isolate FBCC0043) by adopting comparative transcriptomics and functional genomics on solid lignocellulose substrates under varying cultivation atmospheric conditions. Results Switch to fermentative conditions was a major regulator for intracellular metabolism and extracellular enzymatic degradation of wood polysaccharides. Changes in the expression profiles of CAZy (carbohydrate-active enzyme) encoding genes upon oxygen depletion, lead into an alternative wood decomposition strategy. Surprisingly, we noticed higher cellulolytic activity under fermentative conditions in comparison to aerobic cultivation. In addition, our results manifest how oxygen depletion affects over 200 genes of fungal primary metabolism including several transcription factors. We present new functions for acetate generating phosphoketolase pathway and its potential regulator, Adr1 transcription factor, in carbon catabolism under oxygen depletion. Conclusions Physiologically resilient wood-decomposing Basidiomycota species P. radiata is capable of thriving under respirative and fermentative conditions utilizing only untreated lignocellulose as carbon source. Hypoxia-response mechanism in the fungus is, however, divergent from the regulation described for Ascomycota fermenting yeasts or animal-pathogenic species of Basidiomycota.
Subject: 11832 Microbiology and virology
220 Industrial biotechnology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion
Funder: Academy of Finland
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