Assembly of Peripheral Actomyosin Bundles in Epithelial Cells Is Dependent on the CaMKK2/AMPK Pathway

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Rajakylä , E K , Lehtimäki , J I , Acheva , A , Schaible , N , Lappalainen , P , Krishnan , R & Tojkander , S 2020 , ' Assembly of Peripheral Actomyosin Bundles in Epithelial Cells Is Dependent on the CaMKK2/AMPK Pathway ' , Cell Reports , vol. 30 , no. 12 , pp. 4266-4280 . https://doi.org/10.1016/j.celrep.2020.02.096

Title: Assembly of Peripheral Actomyosin Bundles in Epithelial Cells Is Dependent on the CaMKK2/AMPK Pathway
Author: Rajakylä, Eeva Kaisa; Lehtimäki, Jaakko I.; Acheva, Anna; Schaible, Niccole; Lappalainen, Pekka; Krishnan, Ramaswamy; Tojkander, Sari
Contributor: University of Helsinki, Veterinary Biosciences
University of Helsinki, Institute of Biotechnology
University of Helsinki, Veterinary Biosciences
University of Helsinki, Institute of Biotechnology
University of Helsinki, Helsinki One Health (HOH)
Date: 2020-03-24
Language: eng
Number of pages: 19
Belongs to series: Cell Reports
ISSN: 2211-1247
URI: http://hdl.handle.net/10138/314143
Abstract: Summary Defects in the maintenance of intercellular junctions are associated with loss of epithelial barrier function and consequent pathological conditions, including invasive cancers. Epithelial integrity is dependent on actomyosin bundles at adherens junctions, but the origin of these junctional bundles is incompletely understood. Here we show that peripheral actomyosin bundles can be generated from a specific actin stress fiber subtype, transverse arcs, through their lateral fusion at cell-cell contacts. Importantly, we find that assembly and maintenance of peripheral actomyosin bundles are dependent on the mechanosensitive CaMKK2/AMPK signaling pathway and that inhibition of this route leads to disruption of tension-maintaining actomyosin bundles and re-growth of stress fiber precursors. This results in redistribution of cellular forces, defects in monolayer integrity, and loss of epithelial identity. These data provide evidence that the mechanosensitive CaMKK2/AMPK pathway is critical for the maintenance of peripheral actomyosin bundles and thus dictates cell-cell junctions through cellular force distribution.
Subject: epithelia
epithelial integrity
actomyosin bundles
stress fibers
mechanosensing
cellular forces
adhesive structures
actin
myosin
AMPK
CaMKK2
1182 Biochemistry, cell and molecular biology
413 Veterinary science
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