Assembly of Peripheral Actomyosin Bundles in Epithelial Cells Is Dependent on the CaMKK2/AMPK Pathway

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dc.contributor.author Rajakylä, Eeva Kaisa
dc.contributor.author Lehtimäki, Jaakko I.
dc.contributor.author Acheva, Anna
dc.contributor.author Schaible, Niccole
dc.contributor.author Lappalainen, Pekka
dc.contributor.author Krishnan, Ramaswamy
dc.contributor.author Tojkander, Sari
dc.date.accessioned 2020-04-16T10:50:03Z
dc.date.available 2020-04-16T10:50:03Z
dc.date.issued 2020-03-24
dc.identifier.citation Rajakylä , E K , Lehtimäki , J I , Acheva , A , Schaible , N , Lappalainen , P , Krishnan , R & Tojkander , S 2020 , ' Assembly of Peripheral Actomyosin Bundles in Epithelial Cells Is Dependent on the CaMKK2/AMPK Pathway ' , Cell Reports , vol. 30 , no. 12 , pp. 4266-4280 . https://doi.org/10.1016/j.celrep.2020.02.096
dc.identifier.other PURE: 134178233
dc.identifier.other PURE UUID: c18ca7a7-8ebe-4796-a7b3-92f008874c71
dc.identifier.other RIS: urn:F05D873983581AD6B73ED3CA47A33965
dc.identifier.other WOS: 000521989500027
dc.identifier.other ORCID: /0000-0003-1897-4981/work/72304034
dc.identifier.other ORCID: /0000-0001-9447-8977/work/72305983
dc.identifier.uri http://hdl.handle.net/10138/314143
dc.description.abstract Summary Defects in the maintenance of intercellular junctions are associated with loss of epithelial barrier function and consequent pathological conditions, including invasive cancers. Epithelial integrity is dependent on actomyosin bundles at adherens junctions, but the origin of these junctional bundles is incompletely understood. Here we show that peripheral actomyosin bundles can be generated from a specific actin stress fiber subtype, transverse arcs, through their lateral fusion at cell-cell contacts. Importantly, we find that assembly and maintenance of peripheral actomyosin bundles are dependent on the mechanosensitive CaMKK2/AMPK signaling pathway and that inhibition of this route leads to disruption of tension-maintaining actomyosin bundles and re-growth of stress fiber precursors. This results in redistribution of cellular forces, defects in monolayer integrity, and loss of epithelial identity. These data provide evidence that the mechanosensitive CaMKK2/AMPK pathway is critical for the maintenance of peripheral actomyosin bundles and thus dictates cell-cell junctions through cellular force distribution. en
dc.format.extent 19
dc.language.iso eng
dc.relation.ispartof Cell Reports
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject epithelia
dc.subject epithelial integrity
dc.subject actomyosin bundles
dc.subject stress fibers
dc.subject mechanosensing
dc.subject cellular forces
dc.subject adhesive structures
dc.subject actin
dc.subject myosin
dc.subject AMPK
dc.subject CaMKK2
dc.subject 1182 Biochemistry, cell and molecular biology
dc.subject 413 Veterinary science
dc.title Assembly of Peripheral Actomyosin Bundles in Epithelial Cells Is Dependent on the CaMKK2/AMPK Pathway en
dc.type Article
dc.contributor.organization Veterinary Biosciences
dc.contributor.organization Institute of Biotechnology
dc.contributor.organization Veterinary Pathology and Parasitology
dc.contributor.organization Helsinki One Health (HOH)
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.celrep.2020.02.096
dc.relation.issn 2211-1247
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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