Bioprospecting Staphylococcus Phages with Therapeutic and Bio-Control Potential

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Oduor , J M O , Kadija , E , Nyachieo , A , Mureithi , M W & Skurnik , M 2020 , ' Bioprospecting Staphylococcus Phages with Therapeutic and Bio-Control Potential ' , Viruses (Basel) , vol. 12 , no. 2 , 133 .

Title: Bioprospecting Staphylococcus Phages with Therapeutic and Bio-Control Potential
Author: Oduor, Joseph M. Ochieng; Kadija, Ermir; Nyachieo, Atunga; Mureithi, Marianne W.; Skurnik, Mikael
Contributor organization: Faculty of Medicine
Department of Bacteriology and Immunology
HUMI - Human Microbiome Research
Research Programs Unit
University of Helsinki
Helsinki One Health (HOH)
Mikael Skurnik / Principal Investigator
Helsinki University Hospital Area
Date: 2020-02
Language: eng
Number of pages: 13
Belongs to series: Viruses (Basel)
ISSN: 1999-4915
Abstract: Emergence of antibiotic-resistant bacteria is a serious threat to the public health. This is also true for Staphylococcus aureus and other staphylococci. Staphylococcus phages Stab20, Stab21, Stab22, and Stab23, were isolated in Albania. Based on genomic and phylogenetic analysis, they were classified to genus Kayvirus of the subfamily Twortvirinae. In this work, we describe the in-depth characterization of the phages that electron microscopy confirmed to be myoviruses. These phages showed tolerance to pH range of 5.4 to 9.4, to maximum UV radiation energy of 25 mu J/cm(2), to temperatures up to 45 degrees C, and to ethanol concentrations up to 25%, and complete resistance to chloroform. The adsorption rate constants of the phages ranged between 1.0 x 10(-9) mL/min and 4.7 x 10(-9) mL/min, and the burst size was from 42 to 130 plaque-forming units. The phages Stab20, 21, 22, and 23, originally isolated using Staphylococcus xylosus as a host, demonstrated varied host ranges among different Staphylococcus strains suggesting that they could be included in cocktail formulations for therapeutic or bio-control purpose. Phage particle proteomes, consisting on average of ca 60-70 gene products, revealed, in addition to straight-forward structural proteins, also the presence of enzymes such DNA polymerase, helicases, recombinases, exonucleases, and RNA ligase polymer. They are likely to be injected into the bacteria along with the genomic DNA to take over the host metabolism as soon as possible after infection.
Subject: MRSA
3111 Biomedicine
11832 Microbiology and virology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

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