Long-term application of Swedish sewage sludge on farmland does not cause clear changes in the soil bacterial resistome

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dc.contributor.author Rutgersson, Carolin
dc.contributor.author Ebmeyer, Stefan
dc.contributor.author Lassen, Simon Bo
dc.contributor.author Karkman, Antti
dc.contributor.author Fick, Jerker
dc.contributor.author Kristiansson, Erik
dc.contributor.author Brandt, Kristian K.
dc.contributor.author Flach, Carl-Fredrik
dc.contributor.author Larsson, D.G. Joakim
dc.date.accessioned 2020-04-29T13:40:01Z
dc.date.available 2020-04-29T13:40:01Z
dc.date.issued 2020-04
dc.identifier.citation Rutgersson , C , Ebmeyer , S , Lassen , S B , Karkman , A , Fick , J , Kristiansson , E , Brandt , K K , Flach , C-F & Larsson , D G J 2020 , ' Long-term application of Swedish sewage sludge on farmland does not cause clear changes in the soil bacterial resistome ' , Environment International , vol. 137 , 105339 . https://doi.org/10.1016/j.envint.2019.105339
dc.identifier.other PURE: 133167720
dc.identifier.other PURE UUID: 60e8616a-d932-44a7-a9eb-701f720dce7b
dc.identifier.other RIS: urn:4611AF335BDC11F4964773E9F558541D
dc.identifier.other WOS: 000517970800050
dc.identifier.other ORCID: /0000-0003-0983-3319/work/73243697
dc.identifier.uri http://hdl.handle.net/10138/314495
dc.description.abstract The widespread practice of applying sewage sludge to arable land makes use of nutrients indispensable for crops and reduces the need for inorganic fertilizer, however this application also provides a potential route for human exposure to chemical contaminants and microbial pathogens in the sludge. A recent concern is that such practice could promote environmental selection and dissemination of antibiotic resistant bacteria or resistance genes. Understanding the risks of sludge amendment in relation to antibiotic resistance development is important for sustainable agriculture, waste treatment and infectious disease management. To assess such risks, we took advantage of an agricultural field trial in southern Sweden, where land used for growing different crops has been amended with sludge every four years since 1981. We sampled raw, semi-digested and digested and stored sludge together with soils from the experimental plots before and two weeks after the most recent amendment in 2017. Levels of selected antimicrobials and bioavailable metals were determined and microbial effects were evaluated using both culture-independent metagenome sequencing and conventional culturing. Antimicrobials or bioavailable metals (Cu and Zn) did not accumulate to levels of concern for environmental selection of antibiotic resistance, and no coherent signs, neither on short or long time scales, of enrichment of antibiotic-resistant bacteria or resistance genes were found in soils amended with digested and stored sewage sludge in doses up to 12 metric tons per hectare. Likewise, only very few and slight differences in microbial community composition were observed after sludge amendment. Taken together, the current study does not indicate risks of sludge amendment related to antibiotic resistance development under the given conditions. Extrapolations should however be done with care as sludge quality and application practices vary between regions. Hence, the antibiotic concentrations and resistance load of the sludge are likely to be higher in regions with larger antibiotic consumption and resistance burden than Sweden. en
dc.format.extent 12
dc.language.iso eng
dc.relation.ispartof Environment International
dc.rights cc_by_nc_nd
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 4111 Agronomy
dc.subject 11832 Microbiology and virology
dc.subject Digested sludge
dc.subject Antibiotic resistance
dc.subject Agricultural soil
dc.subject Metagenome sequencing
dc.subject Bioavailable metals
dc.subject Bacterial community composition
dc.subject ABUNDANCE
dc.subject AMENDED SOILS
dc.subject METALS
dc.subject FIELD TRIAL
dc.title Long-term application of Swedish sewage sludge on farmland does not cause clear changes in the soil bacterial resistome en
dc.type Article
dc.contributor.organization Department of Microbiology
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1016/j.envint.2019.105339
dc.relation.issn 0160-4120
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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