Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells

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Reigada , I , Pérez-Tanoira , R , Patel , J , Savijoki , K , Yli-Kauhaluoma , J , Kinnari , T & Fallarero , A 2020 , ' Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells ' , Microorganisms , vol. 8 , no. 3 , 345 , pp. 1-20 . https://doi.org/10.3390/microorganisms8030345

Title: Strategies to Prevent Biofilm Infections on Biomaterials: Effect of Novel Naturally-Derived Biofilm Inhibitors on a Competitive Colonization Model of Titanium by Staphylococcus aureus and SaOS-2 Cells
Author: Reigada, Inés; Pérez-Tanoira, Ramon; Patel, Jayendra; Savijoki, Kirsi; Yli-Kauhaluoma, Jari; Kinnari, Teemu; Fallarero, Adyary
Contributor: University of Helsinki, Pharmaceutical Design and Discovery group
University of Helsinki, Pharmaceutical Design and Discovery group
University of Helsinki, Division of Pharmaceutical Biosciences
University of Helsinki, Pharmaceutical Design and Discovery group
University of Helsinki, HUS Head and Neck Center
University of Helsinki, Divisions of Faculty of Pharmacy
Date: 2020-02-29
Language: eng
Number of pages: 20
Belongs to series: Microorganisms
ISSN: 2076-2607
URI: http://hdl.handle.net/10138/314641
Abstract: Biofilm-mediated infection is a major cause of bone prosthesis failure. The lack of molecules able to act in biofilms has driven research aimed at identifying new anti-biofilm agents via chemical screens. However, to be able to accommodate a large number of compounds, the testing conditions of these screenings end up being typically far from the clinical scenario. In this study, we assess the potential applicability of three previously discovered anti-biofilm compounds to be part of implanted medical devices by testing them on in vitro systems that more closely resemble the clinical scenario. To that end, we used a competition model based on the co-culture of SaOS-2 mammalian cells and Staphylococcus aureus (collection and clinical strains) on a titanium surface, as well as titanium pre-conditioned with high serum protein concentration. Additionally, we studied whether these compounds enhance the previously proven protective effect of pre-incubating titanium with SaOS-2 cells. Out of the three, DHA1 was the one with the highest potential, showing a preventive effect on bacterial adherence in all tested conditions, making it the most promising agent for incorporation into bone implants. This study emphasizes and demonstrates the importance of using meaningful experimental models, where potential antimicrobials ought to be tested for the protection of biomaterials in translational applications.
Subject: ADHESION
BACITRACIN
BACTERIA
COATINGS
D-AMINO ACIDS
IMPLANTS
INTEGRATION
SURFACE
SaOS-2
Staphylococcus aureus
biofilm
biomaterials
co-culture
implanted devices
11832 Microbiology and virology
1182 Biochemistry, cell and molecular biology
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