MUC20 expression marks the receptive phase of the human endometrium

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http://hdl.handle.net/10138/314747

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Stepanjuk , A , Koel , M , Pook , M , Saare , M , Jääger , K , Peters , M , Krjutskov , K , Ingerpuu , S & Salumets , A 2019 , ' MUC20 expression marks the receptive phase of the human endometrium ' , Reproductive BioMedicine Online , vol. 39 , no. 5 , pp. 725-736 . https://doi.org/10.1016/j.rbmo.2019.05.004

Title: MUC20 expression marks the receptive phase of the human endometrium
Author: Stepanjuk, Artjom; Koel, Mariann; Pook, Martin; Saare, Merli; Jääger, Kersti; Peters, Maire; Krjutskov, Kaarel; Ingerpuu, Sulev; Salumets, Andres
Contributor: University of Helsinki, CAN-PRO - Translational Cancer Medicine Program
University of Helsinki, HUS Gynecology and Obstetrics
Date: 2019-11
Language: eng
Number of pages: 12
Belongs to series: Reproductive BioMedicine Online
ISSN: 1472-6483
URI: http://hdl.handle.net/10138/314747
Abstract: Research question: How does mucin MUC20 expression change during the menstrual cycle in different cell types of human endometrium? Design: Study involved examination of MUC20 expression in two previously published RNA-seq datasets in whole endometrial tissue (n = 10), sorted endometrial epithelial (n = 44) or stromal (n = 42) cell samples. RNA-Seq results were validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in whole tissue (n = 10), sorted epithelial (n = 17) and stromal (n = 17) cell samples. MUC20 protein localization and expression were analysed in human endometrium by immunohistochemical analysis of intact endometrial tissue (n = 6) and also Western blot of cultured stromal and epithelial cells (n = 2). Results: MUC20 is differentially expressed in the endometrium between the pre-receptive and receptive phases. We show that MUC20 is predominantly expressed by epithelial cells of the receptive endometrium, both at the mRNA (RNA-Seq, P = 0.005; qRT-PCR, P = 0.039) and protein levels (Western blot; immunohistochemistry, P = 0.029). Conclusion: Our results indicate MUC20 as a novel marker of mid-secretory endometrial biology. We propose a model of MUC20 function in the hepatocyte growth factor (HGF)-activated mesenchymal-epithelial transition (MET) receptor signalling specifically in the receptive phase. Further investigations should reveal the precise function of MUC20 in human endometrium and the possible connection between MUC20 and HGF-activated MET receptor signalling. MUC20 could potentially be included in the list of endometrial receptivity markers after further clinical validation.
Subject: MUC20
Endometrium
Implantation
Mucin
Receptivity
Window of implantation
SINGLE-CELL TRANSCRIPTOME
GENE-EXPRESSION
DIFFERENTIAL EXPRESSION
STROMAL CELLS
IDENTIFICATION
IMPLANTATION
SURFACE
BARRIER
TRANSITION
PATHWAY
3123 Gynaecology and paediatrics
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