Impact of JNK and Its Substrates on Dendritic Spine Morphology

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Komulainen , E , Varidaki , A , Kulesskaya , N , Mohammad , H , Sourander , C , Rauvala , H & Coffey , E T 2020 , ' Impact of JNK and Its Substrates on Dendritic Spine Morphology ' , Cells , vol. 9 , no. 2 , 440 . https://doi.org/10.3390/cells9020440

Title: Impact of JNK and Its Substrates on Dendritic Spine Morphology
Author: Komulainen, Emilia; Varidaki, Artemis; Kulesskaya, Natalia; Mohammad, Hasan; Sourander, Christel; Rauvala, Heikki; Coffey, Eleanor T.
Contributor: University of Helsinki, Biosciences
University of Helsinki, Neuroscience Center
Date: 2020-02
Language: eng
Number of pages: 16
Belongs to series: Cells
ISSN: 2073-4409
URI: http://hdl.handle.net/10138/314796
Abstract: The protein kinase JNK1 exhibits high activity in the developing brain, where it regulates dendrite morphology through the phosphorylation of cytoskeletal regulatory proteins. JNK1 also phosphorylates dendritic spine proteins, and Jnk1-/- mice display a long-term depression deficit. Whether JNK1 or other JNKs regulate spine morphology is thus of interest. Here, we characterize dendritic spine morphology in hippocampus of mice lacking Jnk1-/- using Lucifer yellow labelling. We find that mushroom spines decrease and thin spines increase in apical dendrites of CA3 pyramidal neurons with no spine changes in basal dendrites or in CA1. Consistent with this spine deficit, Jnk1-/- mice display impaired acquisition learning in the Morris water maze. In hippocampal cultures, we show that cytosolic but not nuclear JNK, regulates spine morphology and expression of phosphomimicry variants of JNK substrates doublecortin (DCX) or myristoylated alanine-rich C kinase substrate-like protein-1 (MARCKSL1), rescue mushroom, thin, and stubby spines differentially. These data suggest that physiologically active JNK controls the equilibrium between mushroom, thin, and stubby spines via phosphorylation of distinct substrates.
Subject: JNK1
JNK
synaptic plasticity
dendritic spine
substrate
hippocampus
DCX
MARCKSL1
MRP
Morris water maze
N-TERMINAL KINASE
MORRIS WATER MAZE
DOUBLECORTIN
MEMORY
DEPRESSION
PLASTICITY
STRESS
PHOSPHORYLATION
POTENTIATION
1182 Biochemistry, cell and molecular biology
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