Design and synthesis of lipid-mimetic cationic iridium complexes and their liposomal formulation for in vitro and in vivo application in luminescent bioimaging

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dc.contributor.author Shakirova, Julia R.
dc.contributor.author Sadeghi, Amir
dc.contributor.author Koblova, Alla A.
dc.contributor.author Chelushkin, Pavel S.
dc.contributor.author Toropainen, Elisa
dc.contributor.author Tavakoli, Shirin
dc.contributor.author Kontturi, Leena-Stiina
dc.contributor.author Lajunen, Tatu
dc.contributor.author Tunik, Sergey P.
dc.contributor.author Urtti, Arto
dc.date.accessioned 2020-05-19T12:23:03Z
dc.date.available 2020-05-19T12:23:03Z
dc.date.issued 2020-04-07
dc.identifier.citation Shakirova , J R , Sadeghi , A , Koblova , A A , Chelushkin , P S , Toropainen , E , Tavakoli , S , Kontturi , L-S , Lajunen , T , Tunik , S P & Urtti , A 2020 , ' Design and synthesis of lipid-mimetic cationic iridium complexes and their liposomal formulation for in vitro and in vivo application in luminescent bioimaging ' , RSC Advances , vol. 10 , no. 24 , pp. 14431-14440 . https://doi.org/10.1039/d0ra01114b
dc.identifier.other PURE: 137396893
dc.identifier.other PURE UUID: 33956b40-1e63-4720-9214-4bb7bf830060
dc.identifier.other WOS: 000528043700062
dc.identifier.other ORCID: /0000-0001-6234-9193/work/75463556
dc.identifier.other ORCID: /0000-0002-3766-3006/work/75565502
dc.identifier.uri http://hdl.handle.net/10138/315044
dc.description.abstract Two iridium [Ir(NC)(2)(NN)](+) complexes with the diimine NN ligand containing a long polymethylene hydrophobic chain were synthesized and characterized by using NMR and ESI mass-spectrometry: NN - 2-(1-hexadecyl-1H-imidazol-2-yl)pyridine, NC - methyl-2-phenylquinoline-4-carboxylate (Ir1) and 2-phenylquinoline-4-carboxylic acid (Ir2). These complexes were used to prepare the luminescent PEGylated DPPC liposomes (DPPC/DSPE-PEG2000/Ir-complex = 95/4.5/1 mol%) using a thin film hydration method. The narrowly dispersed liposomes had diameters of about 110 nm. The photophysics of the complexes and labeled liposomes were carefully studied. Ir1 and Ir2 give red emission (lambda(em) = 667 and 605 nm) with a lifetime in the microsecond domain and quantum yields of 4.8% and 10.0% in degassed solution. Incorporation of the complexes into the liposome lipid bilayer results in shielding of the emitters from interaction with molecular oxygen and partial suppression of excited state nonradiative relaxation due to the effect of the relatively rigid bilayer matrix. Delivery of labeled liposomes to the cultured ARPE-19 cells demonstrated the usefulness of Ir1 and Ir2 in cellular imaging. Labeled liposomes were then injected intravitreally into rat eyes and imaged successfully with optical coherence tomography and funduscopy. In conclusion, iridium complexes enabled the successful labeling and imaging of liposomes in cells and animals. en
dc.format.extent 10
dc.language.iso eng
dc.relation.ispartof RSC Advances
dc.rights cc_by_nc
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject OPTICAL COHERENCE TOMOGRAPHY
dc.subject DRUG-DELIVERY
dc.subject PHOTOPHYSICAL PROPERTIES
dc.subject LOADED LIPOSOMES
dc.subject FLUORESCENT
dc.subject LIGANDS
dc.subject PEG
dc.subject 116 Chemical sciences
dc.subject 317 Pharmacy
dc.title Design and synthesis of lipid-mimetic cationic iridium complexes and their liposomal formulation for in vitro and in vivo application in luminescent bioimaging en
dc.type Article
dc.contributor.organization Drug Delivery Unit
dc.contributor.organization Division of Pharmaceutical Biosciences
dc.contributor.organization Drug Research Program
dc.contributor.organization Helsinki Institute of Life Science HiLIFE
dc.contributor.organization Pharmaceutical Nanotechnology
dc.contributor.organization Drug Delivery
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1039/d0ra01114b
dc.relation.issn 2046-2069
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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