GNAS, PDE4D, and PRKAR1A Mutations and GNAS Methylation Changes Are Not a Common Cause of Isolated Early-Onset Severe Obesity Among Finnish Children

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Loid , P , Pekkinen , M , Reyes , M , Mustila , T , Viljakainen , H , Jüppner , H & Mäkitie , O 2020 , ' GNAS, PDE4D, and PRKAR1A Mutations and GNAS Methylation Changes Are Not a Common Cause of Isolated Early-Onset Severe Obesity Among Finnish Children ' , Frontiers in pediatrics , vol. 8 , 145 . https://doi.org/10.3389/fped.2020.00145

Title: GNAS, PDE4D, and PRKAR1A Mutations and GNAS Methylation Changes Are Not a Common Cause of Isolated Early-Onset Severe Obesity Among Finnish Children
Author: Loid, Petra; Pekkinen, Minna; Reyes, Monica; Mustila, Taina; Viljakainen, Heli; Jüppner, Harald; Mäkitie, Outi
Contributor: University of Helsinki, HUS Children and Adolescents
University of Helsinki, HUS Children and Adolescents
University of Helsinki, Department of Food and Nutrition
University of Helsinki, HUS Children and Adolescents
Date: 2020-04-07
Language: eng
Number of pages: 5
Belongs to series: Frontiers in pediatrics
ISSN: 2296-2360
URI: http://hdl.handle.net/10138/315435
Abstract: Context: Pseudohypoparathyroidism type Ia (PHP1A) is caused by inactivating mutations involving GNAS exons 1–13, encoding the alpha-subunit of the stimulatory G protein (Gsα). Particularly PHP1A, but also other disorders involving the Gsα-cAMP-signaling pathway, have been associated with early-onset obesity. Thus, patients with mutations in the genes encoding PDE4D and PRKAR1A can also be obese. Furthermore, epigenetic GNAS changes, as in pseudohypoparathyroidism type Ib (PHP1B), can lead to excessive weight.Objective: Search for genetic variants in GNAS, PDE4D, and PRKAR1A and for methylation alterations at the GNAS locus in Finnish subjects with isolated severe obesity before age 10 years.Methods: Next generation sequencing to identify pathogenic variants in the coding exons of GNAS, PDE4D, and PRKAR1A; Multiplex Ligation-dependent Probe Amplification (MLPA) and methylation-sensitive MLPA (MS-MLPA) to search for deletions in GNAS and STX16, and for epigenetic changes at the four differentially methylated regions (DMR) within GNAS.Results: Among the 88 subjects (median age 13.8 years, median body mass index Z-score +3.9), we identified one rare heterozygous missense variant of uncertain significance in the XL exon of GNAS in a single patient. We did not identify clearly pathogenic variants in PDE4D and PRKAR1A, and no GNAS methylation changes were detected by MS-MLPA.Conclusions: Our results suggest that coding GNAS mutations or methylation changes at the GNAS DMRs, or coding mutations in PDE4D and PRKAR1A are not common causes of isolated childhood obesity in Finland.
Subject: 3123 Gynaecology and paediatrics
GNAS
G protein-cAMP-signaling
childhood-onset obesity
pseudohypoparathyroidism
acrodysostosis
PSEUDOHYPOPARATHYROIDISM
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