Diversity and Complexity of the Large Surface Protein Family in the Compacted Genomes of Multiple Pneumocystis Species

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dc.contributor.author Ma, Liang
dc.contributor.author Chen, Zehua
dc.contributor.author Huang, Da Wei
dc.contributor.author Cisse, Ousmane H.
dc.contributor.author Rothenburger, Jamie L.
dc.contributor.author Latinne, Alice
dc.contributor.author Bishop, Lisa
dc.contributor.author Blair, Robert
dc.contributor.author Brenchley, Jason M.
dc.contributor.author Chabe, Magali
dc.contributor.author Deng, Xilong
dc.contributor.author Hirsch, Vanessa
dc.contributor.author Keesler, Rebekah
dc.contributor.author Kutty, Geetha
dc.contributor.author Liu, Yueqin
dc.contributor.author Margolis, Daniel
dc.contributor.author Morand, Serge
dc.contributor.author Pahar, Bapi
dc.contributor.author Peng, Li
dc.contributor.author Van Rompay, Koen K. A.
dc.contributor.author Song, Xiaohong
dc.contributor.author Song, Jun
dc.contributor.author Sukura, Antti
dc.contributor.author Thapar, Sabrina
dc.contributor.author Wang, Honghui
dc.contributor.author Weissenbacher-Lang, Christiane
dc.contributor.author Xu, Jie
dc.contributor.author Lee, Chao-Hung
dc.contributor.author Jardine, Claire
dc.contributor.author Lempicki, Richard A.
dc.contributor.author Cushion, Melanie T.
dc.contributor.author Cuomo, Christina A.
dc.contributor.author Kovacs, Joseph A.
dc.date.accessioned 2020-06-02T10:47:02Z
dc.date.available 2020-06-02T10:47:02Z
dc.date.issued 2020-03-03
dc.identifier.citation Ma , L , Chen , Z , Huang , D W , Cisse , O H , Rothenburger , J L , Latinne , A , Bishop , L , Blair , R , Brenchley , J M , Chabe , M , Deng , X , Hirsch , V , Keesler , R , Kutty , G , Liu , Y , Margolis , D , Morand , S , Pahar , B , Peng , L , Van Rompay , K K A , Song , X , Song , J , Sukura , A , Thapar , S , Wang , H , Weissenbacher-Lang , C , Xu , J , Lee , C-H , Jardine , C , Lempicki , R A , Cushion , M T , Cuomo , C A & Kovacs , J A 2020 , ' Diversity and Complexity of the Large Surface Protein Family in the Compacted Genomes of Multiple Pneumocystis Species ' , mBio , vol. 11 , no. 2 , e02878-19 . https://doi.org/10.1128/mBio.02878-19
dc.identifier.other PURE: 138113375
dc.identifier.other PURE UUID: 3a7dbf21-f186-4bd8-a017-eaaecd024641
dc.identifier.other WOS: 000531071300076
dc.identifier.other ORCID: /0000-0002-8992-1695/work/75942728
dc.identifier.uri http://hdl.handle.net/10138/315702
dc.description.abstract Pneumocystis, a major opportunistic pathogen in patients with a broad range of immunodeficiencies, contains abundant surface proteins encoded by a multicopy gene family, termed the major surface glycoprotein (Msg) gene superfamily. This superfamily has been identified in all Pneumocystis species characterized to date, highlighting its important role in Pneumocystis biology. In this report, through a comprehensive and in-depth characterization of 459 msg genes from 7 Pneurnocystis species, we demonstrate, for the first time, the phylogeny and evolution of conserved domains in Msg proteins and provide a detailed description of the classification, unique characteristics, and phylogenetic relatedness of five Msg families. We further describe, for the first time, the relative expression levels of individual msg families in two rodent Pneumocystis species, the substantial variability of the msg repertoires in P. coda from laboratory and wild rats, and the distinct features of the expression site for the classic msg genes in Pneumocystis from 8 mammalian host species. Our analysis suggests multiple functions for this superfamily rather than just conferring antigenic variation to allow immune evasion as previously believed. This study provides a rich source of information that lays the foundation for the continued experimental exploration of the functions of the Msg superfamily in Pneumocystis biology. IMPORTANCE Pneumocystis continues to be a major cause of disease in humans with immunodeficiency, especially those with HIV/AIDS and organ transplants, and is being seen with increasing frequency worldwide in patients treated with immunode-pleting monoclonal antibodies. Annual health care associated with Pneumocystis pneumonia costs similar to$475 million dollars in the United States alone. In addition to causing overt disease in immunodeficient individuals, Pneumocystis can cause subclinical infection or colonization in healthy individuals, which may play an important role in species preservation and disease transmission. Our work sheds new light on the diversity and complexity of the msg superfamily and strongly suggests that the versatility of this superfamily reflects multiple functions, including antigenic variation to allow immune evasion and optimal adaptation to host environmental conditions to promote efficient infection and transmission. These findings are essential to consider in developing new diagnostic and therapeutic strategies. en
dc.format.extent 20
dc.language.iso eng
dc.relation.ispartof mBio
dc.rights cc_by
dc.rights.uri info:eu-repo/semantics/openAccess
dc.subject 11832 Microbiology and virology
dc.subject 1184 Genetics, developmental biology, physiology
dc.subject classification
dc.subject conserved domains
dc.subject major surface glycoprotein
dc.subject phylogenetic analysis
dc.subject Pneumocystis
dc.subject SP NOV.
dc.subject CARINII
dc.subject GENES
dc.subject SEQUENCE
dc.subject RABBITS
dc.title Diversity and Complexity of the Large Surface Protein Family in the Compacted Genomes of Multiple Pneumocystis Species en
dc.type Article
dc.contributor.organization Veterinary Biosciences
dc.contributor.organization Helsinki One Health (HOH)
dc.contributor.organization Antti Sukura / Principal Investigator
dc.contributor.organization Veterinary Pathology and Parasitology
dc.description.reviewstatus Peer reviewed
dc.relation.doi https://doi.org/10.1128/mBio.02878-19
dc.relation.issn 2150-7511
dc.rights.accesslevel openAccess
dc.type.version publishedVersion

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