Using Microcystin Gene Copies to Determine Potentially-Toxic Blooms, Example from a Shallow Eutrophic Lake Peipsi

Show full item record



Permalink

http://hdl.handle.net/10138/315773

Citation

Panksep , K , Tamm , M , Mantzouki , E , Rantala-Ylinen , A , Laugaste , R , Sivonen , K , Tammeorg , O & Kisand , V 2020 , ' Using Microcystin Gene Copies to Determine Potentially-Toxic Blooms, Example from a Shallow Eutrophic Lake Peipsi ' , Toxins , vol. 12 , no. 4 , 211 . https://doi.org/10.3390/toxins12040211

Title: Using Microcystin Gene Copies to Determine Potentially-Toxic Blooms, Example from a Shallow Eutrophic Lake Peipsi
Author: Panksep, Kristel; Tamm, Marju; Mantzouki, Evanthia; Rantala-Ylinen, Anne; Laugaste, Reet; Sivonen, Kaarina; Tammeorg, Olga; Kisand, Veljo
Contributor: University of Helsinki, Institute of Biotechnology
University of Helsinki, Department of Microbiology
University of Helsinki, Lake Ecosystem Dynamics
Date: 2020-04
Language: eng
Number of pages: 21
Belongs to series: Toxins
ISSN: 2072-6651
URI: http://hdl.handle.net/10138/315773
Abstract: Global warming, paired with eutrophication processes, is shifting phytoplankton communities towards the dominance of bloom-forming and potentially toxic cyanobacteria. The ecosystems of shallow lakes are especially vulnerable to these changes. Traditional monitoring via microscopy is not able to quantify the dynamics of toxin-producing cyanobacteria on a proper spatio-temporal scale. Molecular tools are highly sensitive and can be useful as an early warning tool for lake managers. We quantified the potential microcystin (MC) producers in Lake Peipsi using microscopy and quantitative polymerase chain reaction (qPCR) and analysed the relationship between the abundance of the mcyE genes, MC concentration, MC variants and toxin quota per mcyE gene. We also linked environmental factors to the cyanobacteria community composition. In Lake Peipsi, we found rather moderate MC concentrations, but microcystins and microcystin-producing cyanobacteria were widespread across the lake. Nitrate (NO3-) was a main driver behind the cyanobacterial community at the beginning of the growing season, while in late summer it was primarily associated with the soluble reactive phosphorus (SRP) concentration. A positive relationship was found between the MC quota per mcyE gene and water temperature. The most abundant variant-MC-RR-was associated with MC quota per mcyE gene, while other MC variants did not show any significant impact.
Subject: cyanobacteria
qPCR
mcyE
microcystins
MC quota
Lake Peipsi
HARMFUL CYANOBACTERIAL BLOOMS
CLIMATE-CHANGE
WATER-QUALITY
PHOSPHORUS
PCR
DYNAMICS
PHYTOPLANKTON
ASSOCIATIONS
ZOOPLANKTON
TEMPERATURE
11832 Microbiology and virology
1172 Environmental sciences
Rights:


Files in this item

Total number of downloads: Loading...

Files Size Format View
toxins_12_00211_v2.pdf 3.538Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record