Apoplastic Hydrogen Peroxide in the Growth Zone of the Maize Primary Root. Increased Levels Differentially Modulate Root Elongation under Well-Watered and Water-Stressed Conditions

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Voothuluru , P , Mäkelä , P , Zhu , J , Yamaguchi , M , Oliver , M J , Simmonds , J & Sharp , R 2020 , ' Apoplastic Hydrogen Peroxide in the Growth Zone of the Maize Primary Root. Increased Levels Differentially Modulate Root Elongation under Well-Watered and Water-Stressed Conditions ' , Frontiers in plant science , vol. 11 , 392 , pp. 1-18 . https://doi.org/10.3389/fpls.2020.00392

Title: Apoplastic Hydrogen Peroxide in the Growth Zone of the Maize Primary Root. Increased Levels Differentially Modulate Root Elongation under Well-Watered and Water-Stressed Conditions
Author: Voothuluru, Priya; Mäkelä, Pirjo; Zhu, Jinming; Yamaguchi, Mineo; Oliver, Melvin J.; Simmonds, John; Sharp, Robert
Contributor organization: Department of Agricultural Sciences
Helsinki Institute of Sustainability Science (HELSUS)
Plant Production Sciences
Crop Science Research Group
Date: 2020-04-21
Language: eng
Number of pages: 18
Belongs to series: Frontiers in plant science
ISSN: 1664-462X
DOI: https://doi.org/10.3389/fpls.2020.00392
URI: http://hdl.handle.net/10138/315777
Abstract: Reactive oxygen species (ROS) can act as signaling molecules involved in the acclimation of plants to various abiotic and biotic stresses. However, it is not clear how the generalized increases in ROS and downstream signaling events that occur in response to stressful conditions are coordinated to modify plant growth and development. Previous studies of maize (Zea mays L.) primary root growth under water deficit stress showed that cell elongation is maintained in the apical region of the growth zone but progressively inhibited further from the apex, and that the rate of cell production is also decreased. It was observed that apoplastic ROS, particularly hydrogen peroxide (H2O2), increased specifically in the apical region of the growth zone under water stress, resulting at least partly from increased oxalate oxidase activity in this region. To assess the function of the increase in apoplastic H2O2 in root growth regulation, transgenic maize lines constitutively expressing a wheat oxalate oxidase were utilized in combination with kinematic growth analysis to examine effects of increased apoplastic H2O2 on the spatial pattern of cell elongation and on cell production in well-watered and water-stressed roots. Effects of H2O2 removal (via scavenger pretreatment) specifically from the apical region of the growth zone were also assessed. The results show that apoplastic H2O2 positively modulates cell production and root elongation under well-watered conditions, whereas the normal increase in apoplastic H2O2 in water-stressed roots is causally related to down-regulation of cell production and root growth inhibition. The effects on cell production were accompanied by changes in spatial profiles of cell elongation and in the length of the growth zone. However, effects on overall cell elongation, as reflected in final cell lengths, were minor. These results reveal a fundamental role of apoplastic H2O2 in regulating cell production and root elongation in both well-watered and water-stressed conditions.
Subject: 4111 Agronomy
cell elongation
cell production
root growth
hydrogen peroxide
kinematics
reactive oxygen species
water stress
Zea mays
INCREASED PROLINE DEPOSITION
ABSCISIC-ACID ACCUMULATION
BORER OSTRINIA-NUBILALIS
CELL-DIVISION RATES
SPATIAL-DISTRIBUTION
OXALATE OXIDASE
DROUGHT TOLERANCE
ARABIDOPSIS ROOT
APICAL MERISTEM
OSMOTIC-STRESS
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion


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