An Oral Rinse Active Matrix Metalloproteinase-8 Point-of-Care Immunotest May Be Less Accurate in Patients with Crohn's Disease

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Rautava , J , Gürsoy , U K , Kullström , A , Kononen , E , Sorsa , T , Tervahartiala , T & Gürsoy , M 2020 , ' An Oral Rinse Active Matrix Metalloproteinase-8 Point-of-Care Immunotest May Be Less Accurate in Patients with Crohn's Disease ' , Biomolecules , vol. 10 , no. 3 , 395 . https://doi.org/10.3390/biom10030395

Title: An Oral Rinse Active Matrix Metalloproteinase-8 Point-of-Care Immunotest May Be Less Accurate in Patients with Crohn's Disease
Author: Rautava, Jaana; Gürsoy, Ulvi K.; Kullström, Adrian; Kononen, Eija; Sorsa, Timo; Tervahartiala, Taina; Gürsoy, Mervi
Other contributor: University of Helsinki, University of Turku
University of Helsinki, HUS Head and Neck Center
University of Helsinki, HUS Head and Neck Center






Date: 2020-03
Language: eng
Number of pages: 11
Belongs to series: Biomolecules
ISSN: 2218-273X
DOI: https://doi.org/10.3390/biom10030395
URI: http://hdl.handle.net/10138/315800
Abstract: The diagnostic accuracy of point-of-care (PoC) applications may be compromised in individuals with additional inflammatory conditions. This cross-sectional study examined the performance of a commercial oral rinse active matrix metalloproteinase-8 (aMMP-8) PoC immunotest in individuals with (n = 47) and without Crohn's disease (CD) (n = 41). Oral rinse collected from the participants was analyzed by the PoC immunotest. Molecular forms and fragments of salivary MMP-8 were detected by western immunoblotting. The sensitivity of the immunotest for periodontitis was 60.0% in the CD group and 90.0% in the control group. The respective specificity was 75.0% and 80.0%. In both groups, clinical diagnosis of periodontitis exhibited a significant association with the immunotest results, however, the odds ratio (OR) was more than ten-fold in controls (OR 54.3, 95% CI: 3.1-953, p = 0.006) in comparison to CD patients (OR 5.2, 95% CI: 1.3-21.6, p = 0.022). According to Western immunoblot results, the immunotest MMP-8 positivity was not related to elevated levels of molecular forms and fragments of MMP-8 in the CD group, as in the control group. The diagnostic accuracy of the aMMP-8 PoC oral rinse immunotest is reduced in CD patients, which may be related to lower levels or undetectable complexes.
Subject: collagenases
Crohn's disease
dental caries
mouth
periodontitis
INFLAMMATORY-BOWEL-DISEASE
PERI-IMPLANT DISEASES
2017 WORLD WORKSHOP
PERIODONTAL-DISEASE
SALIVARY BIOMARKERS
CONSENSUS REPORT
CLASSIFICATION
MARKERS
HEALTH
MMP-8
3121 General medicine, internal medicine and other clinical medicine
313 Dentistry
1182 Biochemistry, cell and molecular biology
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