Isolation of Anti-Inflammatory and Epithelium Reinforcing Bacteroides and Parabacteroides Spp. from A Healthy Fecal Donor

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Hiippala , K , Kainulainen , V , Suutarinen , M , Heini , T , Bowers , J R , Jasso-Selles , D , Lemmer , D , Valentine , M , Barnes , R , Engelthaler , D M & Satokari , R 2020 , ' Isolation of Anti-Inflammatory and Epithelium Reinforcing Bacteroides and Parabacteroides Spp. from A Healthy Fecal Donor ' , Nutrients , vol. 12 , no. 4 . https://doi.org/10.3390/nu12040935

Title: Isolation of Anti-Inflammatory and Epithelium Reinforcing Bacteroides and Parabacteroides Spp. from A Healthy Fecal Donor
Author: Hiippala, Kaisa; Kainulainen, Veera; Suutarinen, Maiju; Heini, Tuomas; Bowers, Jolene R.; Jasso-Selles, Daniel; Lemmer, Darrin; Valentine, Michael; Barnes, Riley; Engelthaler, David M.; Satokari, Reetta
Contributor: University of Helsinki, Research Programs Unit
University of Helsinki, Research Programs Unit
University of Helsinki, HUMI - Human Microbiome Research
University of Helsinki, Research Programs Unit
University of Helsinki, Reetta Maria Satokari / Principal Investigator
Date: 2020-04
Language: eng
Number of pages: 18
Belongs to series: Nutrients
ISSN: 2072-6643
URI: http://hdl.handle.net/10138/316184
Abstract: Altered intestinal microbiota is associated with systemic and intestinal diseases, such as inflammatory bowel disease (IBD). Dysbiotic microbiota with enhanced proinflammatory capacity is characterized by depletion of anaerobic commensals, increased proportion of facultatively anaerobic bacteria, as well as reduced diversity and stability. In this study, we developed a high-throughput in vitro screening assay to isolate intestinal commensal bacteria with anti-inflammatory capacity from a healthy fecal microbiota transplantation donor. Freshly isolated gut bacteria were screened for their capacity to attenuate Escherichia coli lipopolysaccharide (LPS)-induced interleukin 8 (IL-8) release from HT-29 cells. The screen yielded a number of Bacteroides and Parabacteroides isolates, which were identified as P. distasonis, B. caccae, B. intestinalis, B. uniformis, B. fragilis, B. vulgatus and B. ovatus using whole genome sequencing. We observed that a cell-cell contact with the epithelium was not necessary to alleviate in vitro inflammation as spent culture media from the isolates were also effective and the anti-inflammatory action did not correlate with the enterocyte adherence capacity of the isolates. The anti-inflammatory isolates also exerted enterocyte monolayer reinforcing action and lacked essential genes to synthetize hexa-acylated, proinflammatory lipid A, part of LPS. Yet, the anti-inflammatory effector molecules remain to be identified. The Bacteroides strains isolated and characterized in this study have potential to be used as so-called next-generation probiotics.
Subject: bacteroides
gut homeostasis
host-microbe interactions
immunomodulation
LPS
next-generation probiotics
OUTER-MEMBRANE VESICLES
MICROBIOTA TRANSPLANTATION
GUT MICROBIOTA
CELLS
COLITIS
SPHINGOLIPIDS
DIVERSITY
ALIGNMENT
ADHESION
3143 Nutrition
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