Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth

Show full item record



Davidson , S , Efremova , M , Riedel , A , Mahata , B , Pramanik , J , Huuhtanen , J , Kar , G , Vento-Tormo , R , Hagai , T , Chen , X , Haniffa , M A , Shields , J D & Teichmann , S A 2020 , ' Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth ' , Cell Reports , vol. 31 , no. 7 , 107628 .

Title: Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth
Author: Davidson, Sarah; Efremova, Mirjana; Riedel, Angela; Mahata, Bidesh; Pramanik, Jhuma; Huuhtanen, Jani; Kar, Gozde; Vento-Tormo, Roser; Hagai, Tzachi; Chen, Xi; Haniffa, Muzlifah A.; Shields, Jacqueline D.; Teichmann, Sarah A.
Contributor organization: Department of Clinical Chemistry and Hematology
University of Helsinki
Helsinki University Hospital Area
Hematologian yksikkö
HUS Comprehensive Cancer Center
Department of Oncology
Date: 2020-05-19
Language: eng
Number of pages: 24
Belongs to series: Cell Reports
ISSN: 2211-1247
Abstract: Here, using single-cell RNA sequencing, we examine the stromal compartment in murine melanoma and draining lymph nodes (LNs) at points across tumor development, providing data at Naive lymphocytes from LNs undergo activation and clonal expansion within the tumor, before PD1 and Lag3 expression, while tumor-associated myeloid cells promote the formation of a suppressive niche. We identify three temporally distinct stromal populations displaying unique functional signatures, conserved across mouse and human tumors. Whereas "immune" stromal cells are observed in early tumors, "contractile" cells become more prevalent at later time points. Complement component C3 is specifically expressed in the immune population. Its cleavage product C3a supports the recruitment of C3aR(+) macrophages, and perturbation of C3a and C3aR disrupts immune infiltration, slowing tumor growth. Our results highlight the power of scRNA-seq to identify complex interplays and increase stromal diversity as a tumor develops, revealing that stromal cells acquire the capacity to modulate immune landscapes from early disease.
1182 Biochemistry, cell and molecular biology
Peer reviewed: Yes
Rights: cc_by
Usage restriction: openAccess
Self-archived version: publishedVersion

Files in this item

Total number of downloads: Loading...

Files Size Format View
PIIS2211124720305817.pdf 8.649Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record