Leskelä , J , Pietiäinen , M , Safer , A , Lehto , M , Metso , J , Malle , E , Buggle , F , Becher , H , Sundvall , J , Grau , A , Pussinen , P J & Palm , F 2020 , ' Serum lipopolysaccharide neutralizing capacity in ischemic stroke. ' , PLoS One , vol. 15 , no. 2 , 0228806 . https://doi.org/10.1371/journal.pone.0228806
Title: | Serum lipopolysaccharide neutralizing capacity in ischemic stroke. |
Author: | Leskelä, Jaakko; Pietiäinen, Milla; Safer, Anton; Lehto, Markku; Metso, Jari; Malle, Ernst; Buggle, Florian; Becher, Heiko; Sundvall, Jouko; Grau, Armin; Pussinen, Pirkko J.; Palm, Frederick |
Contributor organization: | Doctoral Programme in Oral Sciences Department of Oral and Maxillofacial Diseases University of Helsinki HUS Head and Neck Center |
Date: | 2020-02-21 |
Language: | eng |
Number of pages: | 17 |
Belongs to series: | PLoS One |
ISSN: | 1932-6203 |
DOI: | https://doi.org/10.1371/journal.pone.0228806 |
URI: | http://hdl.handle.net/10138/316235 |
Abstract: | Introduction Periodontitis is associated with increased serum lipopolysaccharide (LPS) activity, which may be one mechanism linking periodontitis with the risk of cardiovascular diseases. As LPS-carrying proteins including lipoproteins modify LPS-activity, we investigated the determinants of serum LPS-neutralizing capacity (LPS-NC) in ischemic stroke. The association of LPS-NC and Aggregatibacter actinomycetemcomitans, a major microbial biomarker in periodontitis, was also investigated. Materials and methods The assay to measure LPS-NC was set up by spiking serum samples with E. coli LPS. The LPS-NC, LPS-binding protein (LBP), soluble CD14 (sCD14), lipoprotein profiles, apo(lipoprotein) A-I, apoB, and phospholipid transfer protein (PLTP) activity, were determined in 98 ischemic stroke patients and 100 age- and sex-matched controls. Serum and saliva immune response to A. actinomycetemcomitans, its concentration in saliva, and serotype-distribution were examined. Results LPS-NC values ranged between 51-83% in the whole population. Although several of the LPS-NC determinants differed significantly between cases and controls (PLTP, sCD14, apoA-I, HDL-cholesterol), the levels did not (p = 0.056). The main determinants of LPS-NC were i) triglycerides (beta = -0.68, p Conclusion Serum LPS-NC comprised low PLTP-activity, triglyceride and LDL cholesterol concentrations, as well as high HDL cholesterol and IgG against A. actinomycetemcomitans. The present findings let us to conclude that LPS-NC did not associate with stroke. |
Subject: |
3111 Biomedicine
PHOSPHOLIPID TRANSFER PROTEIN ACTINOBACILLUS-ACTINOMYCETEMCOMITANS PERIODONTAL PATHOGENS CARDIOVASCULAR-DISEASE PLASMA-LIPOPROTEINS ENDOTOXIN ACTIVITY ASSOCIATION INFLAMMATION RELEASE OBESITY |
Peer reviewed: | Yes |
Rights: | cc_by |
Usage restriction: | openAccess |
Self-archived version: | publishedVersion |
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