Serum lipopolysaccharide neutralizing capacity in ischemic stroke.

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Leskelä , J , Pietiäinen , M , Safer , A , Lehto , M , Metso , J , Malle , E , Buggle , F , Becher , H , Sundvall , J , Grau , A , Pussinen , P J & Palm , F 2020 , ' Serum lipopolysaccharide neutralizing capacity in ischemic stroke. ' , PLoS One , vol. 15 , no. 2 , 0228806 . https://doi.org/10.1371/journal.pone.0228806

Title: Serum lipopolysaccharide neutralizing capacity in ischemic stroke.
Author: Leskelä, Jaakko; Pietiäinen, Milla; Safer, Anton; Lehto, Markku; Metso, Jari; Malle, Ernst; Buggle, Florian; Becher, Heiko; Sundvall, Jouko; Grau, Armin; Pussinen, Pirkko J.; Palm, Frederick
Contributor: University of Helsinki, Doctoral Programme in Oral Sciences
University of Helsinki, Department of Oral and Maxillofacial Diseases
University of Helsinki, Folkhälsan Research Center
University of Helsinki, HUS Head and Neck Center
Date: 2020-02-21
Language: eng
Number of pages: 17
Belongs to series: PLoS One
ISSN: 1932-6203
URI: http://hdl.handle.net/10138/316235
Abstract: Introduction Periodontitis is associated with increased serum lipopolysaccharide (LPS) activity, which may be one mechanism linking periodontitis with the risk of cardiovascular diseases. As LPS-carrying proteins including lipoproteins modify LPS-activity, we investigated the determinants of serum LPS-neutralizing capacity (LPS-NC) in ischemic stroke. The association of LPS-NC and Aggregatibacter actinomycetemcomitans, a major microbial biomarker in periodontitis, was also investigated. Materials and methods The assay to measure LPS-NC was set up by spiking serum samples with E. coli LPS. The LPS-NC, LPS-binding protein (LBP), soluble CD14 (sCD14), lipoprotein profiles, apo(lipoprotein) A-I, apoB, and phospholipid transfer protein (PLTP) activity, were determined in 98 ischemic stroke patients and 100 age- and sex-matched controls. Serum and saliva immune response to A. actinomycetemcomitans, its concentration in saliva, and serotype-distribution were examined. Results LPS-NC values ranged between 51-83% in the whole population. Although several of the LPS-NC determinants differed significantly between cases and controls (PLTP, sCD14, apoA-I, HDL-cholesterol), the levels did not (p = 0.056). The main determinants of LPS-NC were i) triglycerides (beta = -0.68, p Conclusion Serum LPS-NC comprised low PLTP-activity, triglyceride and LDL cholesterol concentrations, as well as high HDL cholesterol and IgG against A. actinomycetemcomitans. The present findings let us to conclude that LPS-NC did not associate with stroke.
Subject: 3111 Biomedicine
PHOSPHOLIPID TRANSFER PROTEIN
ACTINOBACILLUS-ACTINOMYCETEMCOMITANS
PERIODONTAL PATHOGENS
CARDIOVASCULAR-DISEASE
PLASMA-LIPOPROTEINS
ENDOTOXIN ACTIVITY
ASSOCIATION
INFLAMMATION
RELEASE
OBESITY
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